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Interference of Mycotoxins with Prenatal Development of the Mouse

II. Ochratoxin a Induced Teratogenic Effects in Relation to the Dose and Stage of Gestation

Mykotoxiners interferens på den prenatala utvecklingen hos mus

II. Ochratoxin Α inducerade teratogena effekter i relation till dos och dråktighetstid

Acta Veterinaria Scandinavica volume 22, pages 535–552 (1981)Cite this article

Abstract

Ochratoxin A was dissolved in corn oil and administered by stomach tube to pregnant CBA mice in varying dosages. On gestation day 8 or 9 the dams were treated with a dose of either 1, 2 or 4 mg/kg body weight and on 1 of various other days (2, 4, 6–7, 10–14, 16) or 2 days prior to conception they were supplied with a dose of 4 mg/kg. The highest amount of the toxin given on day 7, 8 or 9 decreased the prenatal survival and on day 9 diminished the foetal growth. A marked impairment in foetal growth also resulted from treatment before conception. Overt craniofacial anomalies were produced only by exposure on day 8 or 9 and their incidence, multiplicity and severity increased with increasing dosage, the peak effect being on day 9.

A study of the anatomical features of the craniofacial skeleton disclosed the failure of closure of neurocranium and an abnormal configuration, position and size of the bones of the base and lateral walls of the skull.

Sammanfattning

Ochratoxin Α löst i majsolja gavs via magsond i tre olika doser, 1, 2 och 4 mg/kg kroppsvikt, till CBΑ möss på 8:e eller 9:e dräktighetsdygnet. Den högsta dosen gavs dessutom till grupper av möss 2 dygn före eller 2, 4, 6–7, 10–14 och 16 dygn efter konception.

Exponering för högsta dosen under dräktighetsdygn 7, 8 eller 9 orsakade en minskad prenatal överlevnad och vid 9:e dygnet dessutom en reducerad fostertillväxt. En tydligt hämmande effekt på fostertillväxten förelåg även vid behandling före konception. Uppenbara kraniofaciella missbildningar uppkom endast vid exponering dygn 8 eller 9. Missbildningarnas frekvens, multiplicitet och svårighetsgrad var tydligt dosrelaterade. Högsta frekvensen missbildningar uppstod vid behandling dag 9.

Anatomiskt förelåg underutveckling och utebliven slutning av neurokraniet samt onormal konfiguration, position och storlek av benen i skallens basala och laterala väggar.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden

    R. G. Arora & H. Frölén

  2. Swedish Research Institute of National Defence, Department 4, Stockholm, Sweden

    R. G. Arora & H. Frölén

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  1. R. G. Arora
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  2. H. Frölén
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Arora, R.G., Frölén, H. Interference of Mycotoxins with Prenatal Development of the Mouse. Acta Vet Scand 22, 535–552 (1981). https://doi.org/10.1186/BF03548678

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Acta Veterinaria Scandinavica

ISSN: 1751-0147