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Cytokeratins of the Stratum Medium and Stratum Internum of the Equine Hoof Wall in Acute Laminitis

Cytokeratiner i hovväggens stratum internum och stratum internum vid akut fång

Acta Veterinaria Scandinavica volume 41, pages 363–379 (2000)Cite this article

Abstract

The cytoskeleton of living keratinocytes consists mainly of cytokeratins that have polymerised into intermediate filaments. The aim of this study was to describe the expression of cytokeratins in the living epidermal cells of the weight-bearing parts of the equine hoof wall during acute spontaneous laminitis. A total of 9 hooves from 3 horses subjected to euthanasia within 48 h of the first clinical signs of laminitis were sectioned and examined. The cytokeratins in the stratum medium and stratum internum of the hoof wall were characterized by 1- and 2-dimensional gel electrophoresis, and the tissue distribution of the cytokeratins was studied by immunohistochemical staining. The biochemical results showed the same set of cytokeratins as was seen in 8 normal horses, reported on previously, used as controls. The immunohistochemical results indicated a difference between normal horses and horses with acute laminitis in the content of cytokeratins in the basal cells of the matrix of the stratum medium of the hoof wall and in the basal and suprabasal cells in the stratum internum at the mid level of the hoof wall. However, no conclusion could be drawn as to whether this change in the cytokeratin distribution in laminitis was primary or was caused by the initiation of the local tissue-repairing process.

Sammanfattning

Huvudparten av de levande keratinocyternas cytoskelett utgörs av cytokeratiner som har polymeriserat till intermediärfilament. Syftet med denna studie var att, på hästar med akut fång, beskriva uttryckandet av cytokeratiner i de levande epidermala cellerna i hovväggens viktbärande delar. Nio hovar, från 3 hästar, har provtagits inom 48 timmar från första kliniska symtom på akut fång. Cytokeratinerna i hovväggens strata medium och internum karakteriserades med 1-och 2-dimensionell elektrofores och cytokeratinernas vävnadsdistribution studerades med immunohistokemi. De biokemiska resultaten uppvisade inga skillnader mellan de akuta fånghästarna och de 8 kontrollhästarna, däremot indikerade de immunohistokemiska resultaten en skillnad mellan de 2 grupperna i cytokeratinernas vävnadsdistribution i följande celiager: 1) basalceller i matrix till stratum medium 2) basal- och suprabasalceller i stratum internum, i nivå med hovväggens mitt. Det var inte möjligt att avgöra om skillnaderna i cytokeratinernas vävnadsdistribution var primära eller om de var sekundära till följd av en lokal vävnadsregeneration.

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  1. Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7018, S-750 07, Uppsala, Sweden

    O. Wattle

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Wattle, O. Cytokeratins of the Stratum Medium and Stratum Internum of the Equine Hoof Wall in Acute Laminitis. Acta Vet Scand 41, 363–379 (2000). https://doi.org/10.1186/BF03549627

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Acta Veterinaria Scandinavica

ISSN: 1751-0147