Skip to main content
Download PDF
  • Published:

Plasma Erythropoietin in Newborn Lambs

Erytropoietin i plasma hos nyfødte lam

Acta Veterinaria Scandinavica volume 41, pages 381–385 (2000)Cite this article

Abstract

In the present study early postnatal changes in erythropoietin (Epo) level and hemoglobin concentration in 8 lambs were examined. Plasma Epo was estimated by a monoclonal enzyme-immunoassay ( ELISA), developed for human Epo. In all the lambs, except one, Epo was low, or undetectable, immediately after birth. Within 6–12 h after birth, a marked increase in plasma Epo was found in 4 of the lambs. Within 3–7 days after birth, Epo was back to low levels. In the lamb with high Epo levels at birth, drastic decreases were observed during the next 6 h. There was no obvious correlation between the hemoglobin concentrations and the changes in plasma Epo.

Sammendrag

Erytropoietin (Epo) i plasma og blodets hemoglobin-konsentrasjon ble hos 8 lam undersøkt de første dagene etter fødsel. PlasmaEpo ble målt med en monoklonal enzym-immunoassay (Elisa), utviklet for humant Epo. Umiddelbart etter fødsel var Eponivåene i plasma lave, eller ikke målbare, hos alle lammene, untatt ett. I løpet av 6–12 timer etter fødsel økte Epo-nivået betydelig hos 4 av de 7 resterende lammene. Etter 3–7 dager var plasmaEpo igjen nede på et lavt nivå. Hos lammet med høyt Epo ved fødsel, falt nivået drastisk de 6 første timene etter fødselen. Det var ingen tydelig sammenheng mellom hemoglobinkonsentrasjonen og forandringene i plasmaEpo.

References

  • Brown MS, Garcia JF, Phibbs RH, Dallman PR: Decreased response of immunoreactive erythropoietin to “available oxygen” in anemia of prematurity. J. Pediatr. 1984, 105, 793–798.

    Article  CAS  Google Scholar 

  • Caro J, Erslev AJ, Silver R, Miller O,Birgegard G: Erythropoietin production in response to anemia or hypoxia in the newborn rat. Blood. 1982, 60, 984–988.

    Article  CAS  Google Scholar 

  • Comline RS, Silver M: Placental transfer of blood gases. Br Med Bull 1975, Jan; 31(1), 25–31

    Article  CAS  Google Scholar 

  • Eckardt KU, Dittmer J, Neumann R, Bauer Ch, Kurtz A: Decline of erythropoietin formation at continuous hypoxia is not due to feedback inhibition. Am. J. Physiol. 258 (Renal Fluid Electrolyte Physiol. 27). 1990 F1432–F1437.

    CAS  Google Scholar 

  • Halvorsen S, Finne RH: Erythropoietin production in the human fetus and newborn. Ann. N.Y. Acad. Sci. 1968, 149, 576–577.

    Article  CAS  Google Scholar 

  • Hågå P, Meberg A, Halvorsen S: Plasma erythropoietin concentrations during the early anemia of prematurity. Acta. Pediat. Scand. 1983, 72, 827–831.

    Article  Google Scholar 

  • Gross DM, Brookins J, Fink GD, Fisher JW: Effects of prostaglandin A2, E2 and F2a on erythropoietin production. J. Pharmacol. Exp. Ther. 1976, 198, 489–496.

    CAS  Google Scholar 

  • Jelkmann W, Wiedemann G: Serum erythropoietin level: relationships to blood hemoglobin concentration and erythropoietic activity of the bone marrow. Klin. Wochenschr. 1990, 68, 403–407.

    Article  CAS  Google Scholar 

  • Jelkmann W: Erythropoietin: structure, control of production and function. Physiol. Rev. 1992, 72, 449–489.

    Article  CAS  Google Scholar 

  • Lim GB, Darby I, Jeyaseelan K, Wintour EM: Ontogeny and regulation of erythropoietin gene expression in the ovine fetus. Clin. Invest. 1994, 72, B 16.

    Google Scholar 

  • Moritz KM, Lim GB, Wintour EM: Developmental regulation of erythropoietin and erythropoiesis. Am. J. Physiol. 1997, 273, 1829–1844.

    Google Scholar 

  • Paulo LG, Wilkerson RD, Roh BL, George WJ, Fisher JW: The effects of prostaglandin E2 on erythropoietin production. Proc. Soc. Exp. Biol. Med. 1973, 142, 771–775.

    Article  CAS  Google Scholar 

  • Peschle C, Sasso GF, Mastroberandino G, Condorelli M: The mechanism of endocrine influences on erythropoiesis. J. Lab. Clin. Med. 1971, 78, 20–29.

    CAS  Google Scholar 

  • Sjaastad ØV, Framstad T, Blom AK: Neonatal changes in plasma erythropoietin in fast growing pigs. Acta. Vet. Scand. 1992, 37, 133–138.

    Article  Google Scholar 

  • Thomas RM, Canning CE, Cotes PM, Linch DC, Rodeck CH, Rossiter CE, Huehns ER: Erythropoietin and cord blood haemoglobin in the regulation of human fetal erythopoiesis. Brit. J. Obstr. Gynaecol. 1983, 90, 795–800.

    Article  CAS  Google Scholar 

  • Wintour EM, Clemons G, Butkus A,Horvath A, Moritz K Towstoless MK: Immunoreactive erythropoietin concentration and haemoglobin type in the perinatal period in sheep of various haemoglobin genotypes. J. Dev. Physiol. 1990, 14, 259–265.

    CAS  Google Scholar 

  • Zanjani ED, Poster J, Mann LI, Wasserman LR: Regulation of erythropoiesis in the fetus. Kidney Hormones, Vol II. Erythropoietin. Fisher JW. ed. 1977 Academic Press, 463–493.

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Physiolology and Nutrition, The Norwegian School of Veterinary Science, Postboks 8146, Oslo-Dep., N-0033, Oslo, Norway

    Ø. V. Sjaastad & A. K. Blom

  2. Department of Reproduction and Forensic Medicine, The Norwegian School of Veterinary Science, Oslo, Norway

    A-K. Egeli & T. Framstad

Authors
  1. Ø. V. Sjaastad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A-K. Egeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. K. Blom
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Framstad
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sjaastad, Ø.V., Egeli, AK., Blom, A.K. et al. Plasma Erythropoietin in Newborn Lambs. Acta Vet Scand 41, 381–385 (2000). https://doi.org/10.1186/BF03549628

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03549628

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147