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Identification of Escherichia coli Recovered from Milk of Sows with Coliform Mastitis by Random Amplified Polymorphic DNA (RAPD) Using Standardized Reagents

Karakterisering av E.coli stammar isolerade från mjölk från suggor med coliform mastit med hjälp av en Random amplified Polymorphic DNA teknik

Acta Veterinaria Scandinavica volume 41, pages 249–259 (2000)Cite this article

Abstract

A standardized-reagents commercial kit for random amplified polymorphic DNA (RAPD) analysis was used for typing 58 Escherichia coli strains that were recovered from the milk of sows, having coliform mastitis, within a single swineherd in Sweden. Previously, the 58 E. coli strains were characterized serologically and profiled biochemically. They were also evaluated for their serum resistance and their ability to adhere to fibronectin and bovine fetal fibroblasts. The RAPD analysis was fast, easily performed, and required only a nanogram of DNA. The indistinguishable banding patterns obtained with repeated analyses of 2 isolates from each strain demonstrated that RAPD analysis using standardized beads is a technique that provides reproducible results for typing E. coli strains that cause mastitis in sows. The results of the RAPD analyses demonstrated that E. coli sow mastitis strains are highly variable in serotype, biochemical profiles, virulence factors, and RAPD type, and that all 58 strains can be differentiated by means of the RAPD technique. The strains grouped into 24 RAPD types by combining the results of 2 primers, and into 38 groups by combining the results of serotype and RAPD type. No relationship between serotypes, virulence factors and RAPD types was found.

Sammanfattning

En “random amplified polymorpic DNA” (RAPD) teknik utvärderades för subtypning av 58 E. coli isolat från mjölk från suggor med coliform mastit. Resultaten visade en mycket hög variabilitet och alla isolat kunde differentieras genom RAPD tekniken. Detta överenstämmer också med en hög variabilitet i såväl serotyp som biokemiskt mönster hos samma stammar. Sammanfattningsvis förefaller det som E. coli stammar förorsakande mastit hos gris inte är beroende på specifika virulensfaktorer utan snarare på omgivningssmitta sannolikt huvudsakligen förorsakade av E. coli av fekalt ursprung.

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Authors and Affiliations

  1. Section of Bacteriology, Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, P.O. Box 583, S-751 23, Uppsala, Sweden

    P. Ramasoota, K. Krovacek, N. Chansiripornchai & S. B. Svenson

  2. Department of Social and Environmental Medicine, Faculty of Tropical medicine, Mahidol University, Bangkok, Thailand

    P. Ramasoota

  3. Bayer AG, Animal Health, Agricultural Center Monheim, Leverkusen, Germany

    A. Pedersen Mörner

  4. Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

    N. Chansiripornchai

Authors
  1. P. Ramasoota
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  2. K. Krovacek
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  3. N. Chansiripornchai
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  4. A. Pedersen Mörner
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  5. S. B. Svenson
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Correspondence to S. B. Svenson.

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Ramasoota, P., Krovacek, K., Chansiripornchai, N. et al. Identification of Escherichia coli Recovered from Milk of Sows with Coliform Mastitis by Random Amplified Polymorphic DNA (RAPD) Using Standardized Reagents. Acta Vet Scand 41, 249–259 (2000). https://doi.org/10.1186/BF03549634

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Acta Veterinaria Scandinavica

ISSN: 1751-0147