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Listeria monocytogenes in Faeces from Clinically Healthy Dairy Cows in Sweden

Listeria monocytogenes i träckprover från kliniskt friska mjölkkor

Acta Veterinaria Scandinavica volume 41, pages 167–171 (2000)Cite this article

Abstract

Faecal samples from 102 clinically healthy dairy cows, representing 34 farms in the Swedish province of Uppsala, were analysed for the presence of Listeria spp. using an enrichment procedure. Listeria monocytogenes was isolated from six (6%) and L. innocua from 2 (2%) cows. From each of the 6 samples positive for L. monocytogenes, 5 isolates were further characterised by restriction enzyme analysis using the 3 enzymes Apa I, Sma I, and Asc I, followed by pulsed-field gel electrophoresis. Three of the L. monocytogenes positive cows lived at the same farm, and they all harboured the same clonal type. One of these 3 cows also harboured a further clonal type of L. monocytogenes. The fact that one of the cows harboured 2 different clonal types of L. monocytogenes is important from an epidemiological point of view when routes of infection are to be investigated.

Sammendrag

Träckprover från 102 kliniskt friska kor i Uppsala kommun analyserades med avseende på förekomst av Listeria spp. Listeria monocytogenes isolerades från sex och L. innocua från 2 kor. Från varje L. monocytogenes-positivt prov karakteriserades 5 isolat genom restriktionsenzymklyvning med enzymerna Apa I, Sma I och Asc I följt av pulsfältsgelelektrofores. Samma klontyp av L. monocytogenes isolerades från 3 kor från samma gård. Från en av dessa kor isolerades dessutom ytterligare en klontyp.

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Authors and Affiliations

  1. Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, P.O. Box 7009, S-750 07, Uppsala, Sweden

    H. Unnerstad, A. Romell, H. Ericsson, M. L. Danielsson-Tham & W. Tham

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  1. H. Unnerstad
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  2. A. Romell
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  3. H. Ericsson
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  4. M. L. Danielsson-Tham
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  5. W. Tham
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Correspondence to H. Unnerstad.

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Unnerstad, H., Romell, A., Ericsson, H. et al. Listeria monocytogenes in Faeces from Clinically Healthy Dairy Cows in Sweden. Acta Vet Scand 41, 167–171 (2000). https://doi.org/10.1186/BF03549648

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Acta Veterinaria Scandinavica

ISSN: 1751-0147