Validation of an enzyme-linked immunosorbent assay developed for measuring cortisol concentration in human saliva and serum for its applicability to analyze cortisol in pig saliva

Table 1 Recovery rate in three samples from 3 sows (A, B and C) with different cortisol concentration mixed with accompanying standards

Added concentration¹μg/dl	Sow A (0.58 μg/dl)²			Sow B (0.42 μg/dl)²			Sow C (0.54 μg/dl)²
Added concentration¹μg/dl	Expected conc.	Detected conc.	Recovery %	Expected conc.	Detected conc.	Recovery %	Expected conc.	Detected conc.	Recovery %
0.03	0.31	0.37	119	0.23	0.21	91	0.29	0.42	145
0.06	0.32	0.34	106	0.24	0.19	79	0.30	0.48	160
0.20	0.39	0.42	108	0.31	0.24	77	0.56	0.56	151
0.60	0.59	0.57	97	0.51	0.49	96	0.57	0.91	160
1.50	1.04	1.31	126	0.96	0.90	94	1.02	1.78	175
4.00	2.29	2.38	104	2.21	2.08	94	2.27	3.00	132

ISSN: 1751-0147