A dose-finding, long-term study on the use of calcium chloride in saline solution as a method of nonsurgical sterilization in dogs: evaluation of the most effective concentration with the lowest risk

Table 1 Effects on reproductive parameters 1 year after intratesticular injection of calcium chloride or saline

Intratesticular injection	Total sperm count (×10⁶)	Sperm motility (%)	Serum testosterone concentration (ng/dL) after hCG stimulation	Testicular width (mm)
Saline Control (E)	311.4 ± 21.4	80 ± 5.8%	735.2 ± 186.4	24.9 ± 2.1
10% CaCl₂ (A)	29.8 ± 38.7⁺	10 ± 5.2%⁺	487.7 ± 144.2	12.9 ± 1.4
20% CaCl₂ (B)	7.5 ± 16.2⁺⁺	5 ± 2.1%⁺⁺	461.0 ± 286.6	12.3 ± 0.9
30% CaCl₂ (C)	0	-	299.3 ± 170.7	12.4 ± 1.5
60% CaCl₂ (D)	0	-	125.7 ± 48.9	12.0 ± 0.7

Effects on reproductive parameters in male dogs were measured 1 year after a single, bilateral intratesticular injection of calcium chloride. Some of the male dogs in groups C and D also underwent surgical castration. Data for dogs in groups A, B, and E and dogs in group C and D that did not undergo surgical castration are expressed as mean ± SD. +60% of the dogs were azoospermic, and 40% were oligospermic. ++80% of the dogs were azoospermic, and 20% showed severe oligospermia.
- Undetectable.

ISSN: 1751-0147