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Table 1 Quantitative detection of C. botulinum in slug carcasses by qPCR

From: Invasive slug populations (Arion vulgaris) as potential vectors for Clostridium botulinum

Day

N positive

Mean Cqa(SD)

Mean log CFU/g (SD)

3

0 (of 3)

ND

ND

4

1 (of 3)

39.15 (±0.72)

1.4 (±1.1)

5

1 (of 3)

38.39 (±1.01)

1.7 (±1.5)

8

1 (of 3)

38.97 (±0.63)

1.6 (±1.2)

10

1 (of 3)

39.79 (±0.05)

1.3 (±≅ 0)

15

0 (of 3)

ND

ND

17

2 (of 3)

38.9 (±0.07)

1.6 (±0.03)

  1. aMean Cq represents the mean of the positively quantified samples. Each sample was run in three qPCR replicates. The corresponding mean log CFU/g was calculated from the standard curve.
  2. qPCR, Quantitative real-time PCR.
  3. Cq, Quantification cycle.
  4. SD, Standard deviation.
  5. CFU, Colony-forming units.
  6. ND, Not detected; less than 2 replicates per sample yielded Cq values <40.
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Acta Veterinaria Scandinavica

ISSN: 1751-0147