Table 3 Polyclonal antibodies used in immunohistochemical (IHC) and western blot (WB) analyses
CYP-enzyme | Primary anti-body | Company | Dilution |
|---|---|---|---|
CYP1A | Polyclonal rabbit-anti-rat (AB1247) CYP1A1 | Chemicon International, Temecula, CA, USA | WB: 1:2000 |
IHC: 1:1000 | |||
CYP2A | Polyclonal rabbit-anti-human (CR3260) CP2A6 | Enzo Life Sciences, Farmingdale, NY, USA | WB: 1:2000 |
IHC 1: 1000 | |||
CYP2C | Polyclonal sheep-anti-human/rat (CR3285) CYP2C8; 2C9; 2C19; rat 2C12 | Enzo Life Sciences, Farmingdale, NY, USA | WB: 1:1000 |
IHC:1: 4000 | |||
CYP2D | Polyclonal rabbit-anti-rat (CR3210) CYP2D1 | Enzo Life Sciences, Farmingdale, NY, USA | WB: 1:1000 |
IHC: 1:2000 | |||
CYP2E | Polyclonal rabbit-anti-human/rat (CR3271) CYP2E1 | BIOMOL International, LP, USA | WB: 1:1000 |
IHC: 1:2000 |