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Table 2 Analytical methods used on the Dimension ExL

From: Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria

Analyte

Unit

Method

TEo (TEa) (%)

Glucose

mmol/l

hexokinase-G6-PDG

5.0 (20)

Urea

mmol/l

enzymatic-kinetic UV-test with urease

13.1 (15)

Creatinine

μmol/l

picric acid

13.3 (20)

Total protein

g/l

biuret

9.9 (10)

Albumin

g/l

bromocresol green

10.8 (15)

Bilirubin, total

μmol/l

diazo-sulfaniline acid

11.1 (30)

Bilirubin, direct

μmol/l

diazo-sulfaniline acid/HCl

37.3 (35)

Triglycerides

mmol/l

4-step, enzymatic

10.2 (25)

Cholesterol

mmol/l

cholesterol esterase/cholesterol oxidase

6.6 (20)

Alkaline phosphatase (ALP)

μkat/l (IU/l)

IFCC, kinetic

18.9 (25)

Aspartate aminotransferase (AST)

μkat/l (IU/l)

IFCC, with pyridoxale-phosphate activation

9.1 (30)

Alanine aminotransferase (ALT)

μkat/l (IU/l)

IFCC, with pyridoxale phosphate activation

23.0 (25)

Glutamate hydrogenase (GLDH)

μkat/l (IU/l)

DGKC, optimised

45.1 (30)

γ-glutamyl transferase (GGT)

μkat/l (IU/l)

IFCC, kinetic

21.9 (20)

Lipase

μkat/l (IU/l)

DGGR

24.5 (25)

Creatine kinase (CK)

μkat/l (IU/l)

IFCC, kinetic

7.1 (30)

Lactate dehydrogenase (LDH)

μkat/l (IU/l)

IFCC, kinetic

8.7 (20)

Iron (Fe)

μmol/l

Ferene

6.3 (30)

Calcium (Ca)

mmol/l

α-cresolpthaleine

7.5 (10)

Phosphorus (P)

mmol/l

phosphate-molybtate, modified

10.2 (15)

Magnesium (Mg)

mmol/l

methylthymol blue

10.9 (20)

Sodium (Na)

mmol/l

ISE

3.3 (5)

Potassium (K)

mmol/l

ISE

7.3 (5)

Chloride (Cl)

mmol/l

ISE

4.8 (5)

  1. The TEo in bold indicates that the value exceeded the ASVCP-recommended TEa during the period of analysis
  2. DGKG Deutsche Gesellschaft für klinische Chemie, DGGR 1,2-o-dilauryl-rac-glycero glutaric; acid-(6′-methylresorufin), HCl hydrochloric acid, IFCC international federation for clinical chemistry, ISE ion selective electrode, PDG pyruvate dehydrogenase, TEa: total allowable error, TEo total observed error