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Table 4 Reference limits and their confidence intervals for biochemical analytes; n indicates the number of reference individuals after the removal of outliers, CI width ratio describes the width of the lower and upper reference limit in comparison to the width of the RIs

From: Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria

Analyte

Number

Reference interval

CI lower reference limit

CI upper reference limit

CI width

ratio

LRL / URL

Glucose

135

4.0–5.7

3.9–4.1

5.5–5.8

0.1 / 0.2

Urea

139

3.2–6.4

3.0–3.5

6.0–6.7

0.2 / 0.2

Creatinine

137

79.6–141.4

79.6–88.4

132.6–141.4

0.1 / 0.1

Total protein

138

54.4–72.9

53.1–55.5

71.4–75.1

0.1 / 0.2

Albumin

132

27.7–36.8

27.3–28.5

35.4–37.2

0.1 / 0.2

Bilirubin, total

132

8.1–21.1

7.5–9.7

20.2–22.4

0.2 / 0.2

Bilirubin, direct

136

2.7–5.5

2.6–2.9

5.1–5.6

0.1 / 0.1

Triglycerides

133

0.03–0.44

0.01–0.06

0.38–0.47

0.1 / 0.2

Cholesterol

136

1.75–2.90

1.68–1.81

2.77–3.00

0.1 / 0.2

ALP

135

1.35–3.55

1.28–1.52

3.45–3.67

0.1 / 0.1

(81–213)

(77–91)

(207–220)

AST

133

4.52–8.80

3.85–4.82

8.15–8.87

0.2 / 0.2

(271–528)

(231–289)

(489–532)

ALT

138

0.01–0.15

0.0–0.02

0.15–0.17

0.1 / 0.1

(0.5–9.0)

(0.0–1.0)

(9.0–10.0)

GLDH

132

0.0–0.18

0.0–0.002

0.16–0.19

0.0 / 0.2

(0.0–10.7)

(0.0–0.1)

(9.4–11.5)

GGT

127

0.11–0.39

0.06–0.14

0.36–0.40

0.2 / 0.1

(6.6–23.6)

(3.3–8.2)

(21.4–23,8)

Lipase

135

0.43–1.12

0.38–0.47

1.07–1.15

0.1 / 0.1

(26–67)

(23–28)

(64–69)

CK

134

2.53–6.52

2.28–2.72

5.92–6.57

0.1 / 0.2

(152–391)

(137–163)

(355–394)

LDH

136

3.32–7.95

2.80–3.70

7.65–8.17

0.2 / 0.1

(199–477)

(168–222)

(459–490)

Iron (Fe)

137

16.4–39.9

14.7–21.7

38.0–41.4

0.3 / 0.1

Calcium (Ca)

139

2.69–3.19

2.65–2.73

3.16–3.25

0.2 / 0.2

Phosphate (P)

139

0.5–1.3

0.4–0.6

1.3–1.5

0.3 / 0.3

Magnesium (Mg)

138

0.6–0.9

0.6–0.6

0.9–0.9

0.0 / 0.0

Sodium (Na)

138

134–141

134–135

140–141

0.2 / 0.2

Potassium (K)

109

3.6–4.7

3.5–3.6

4.6–4.8

0.1 / 0.2

Chloride (Cl)

133

100–105

100–100

105–105

0.0 / 0.0

  1. Bold: ratio exceeded the desired maximum ratio (<0.2). Enzyme activities in brackets are in IU/l
  2. ALT alanine aminotransferase, AP alkaline phosphatase, ASVCP american society for veterinary clinical pathology, AST aspartate aminotransferase, CK creatine kinase, GGT γ-glutamyl transferase, GLDH glutamate hydrogenase, LDH lactate dehydrogenase, LRL lower reverence limit, RI(s) reference interval(s), URL upper reference limit