Table 2 Primer and probe sequences with their respective dye and quencher used for the qRT-PCR
Primer/probe | Target | Concentration | Sequence 5′-->3′ |
|---|---|---|---|
toxA | |||
 toxA-F | toxA | 0.4 µM | GAAATGGCTGGAAAAACCAGTG |
 toxA-R | toxA | 0.4 µM | GAAAAGGCGCTGAAATTACTGTATC |
 toxA-probe | toxA | 200 nM | 5-FAM-CGGCTGATTTAATACGCTTTGCCTTGC–BHQ-1 |
Internal control | |||
 eGFP-1-F | eGFP | 0.2 µM | GACCACTACCAGCAGAACAC |
 eGFP-10-R | eGFP | 0.2 µM | GAACTCCAGCAGGACCATG |
 eGFP-probe | eGFP | 25 nM | ATTO 647 N-AGCACCCAGTCCGCCCTGAGCA-BHQ-3 |