A novel quantitative real-time polymerase chain reaction method for detecting toxigenic Pasteurella multocida in nasal swabs from swine

Table 3 Determination of the LOD of the qRT-PCR

Target	Genome equivalents P. multocida ATCC 12948	Ct	SD
A. toxA (FAM)	2	37.14	0.77
	5	36.06	0.58
	10	34.74	0.37
	20	33.80	0.37
	40	32.78	0.21
	400	29.30	0.17
	4000	25.91	0.23
B. eGFP (ATTO 647N)	2	28.12	0.15
	5	28.04	0.16
	10	27.98	0.21
	20	28.05	0.19
	40	28.03	0.27
	400	27.99	0.18
	4000	27.94	0.14

Ct values using DNA template of the P. multocida ATCC 12948 reference strain and its standard deviation (SD) of a series of 12 replicates were determined for each dilution step ranging from 2 to 4,000 genome equivalents. A Ct values of the toxA measured by the FAM detection channel are represented whereas in (B) Ct values of the internal amplification control measured by the Cy5 detection channel are shown

ISSN: 1751-0147