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Table 2 Number and proportion of samples with DNA of a target bacterial species or group of species detected alone in 117,511 quarter milk samples analyzed in the laboratory of Valio Ltd. in Finland during 2010–2012 using PathoProofTM Mastitis PCR Assay, and the mean cycle threshold (Ct) values

From: Bacteriological etiology and treatment of mastitis in Finnish dairy herds

Pathogen

N

%

Mean Ct valuea

CNS

50,836

43.3

31.5

 Staphylococcus aureus

24,754

21.1

28.0

 Streptococcus uberis

10,572

9.0

26.6

 Streptococcus dysgalactiae

9292

7.9

27.0

 Corynebacterium bovis

8510

7.2

31.9

 Escherichia coli

5562

4.7

29.3

 Trueperella pyogenes/Peptoniphilus indolicus

2921

2.5

26.0

Yeasts

1812

1.5

31.3

 Enterococcus spp.

1722

1.5

30.7

 Klebsiella spp.

836

0.7

28.9

 Streptococcus agalactiae

501

0.4

25.6

 Serratia marcescens

149

0.1

33.1

 Prototheca sp.

34

0

32.7

 Mycoplasma spp.

7

0

21.9

 Mycoplasma bovis

3

0

27.1

Total

117,511

100

 
  1. aCt value reflects the amount of pathogen DNA in the sample: the lower Ct value, the more DNA