Goats in the city: prevalence of Giardia duodenalis and Cryptosporidium spp. in extensively reared goats in northern India

Table 5 Results from sequencing and analysis of positive polymerase chain reaction products. Giardia assemblages are noted with capital letter before semicolon preceding GenBank Accession number

Area	Sample no	No. of cysts isolated by IMS^a	DAPI (%)^b	GDH^c	TPI^d	Bg^e	SSU^f
Chandigarh
Kansal	1	10	100	–	–	–	B; MF069062
	2	220	30	–	–	–	E; MF069058
	3	10	100	–	–	–	A; MF069057
	4	20	100	–	–	–	B; MF069047
	5	100	40	–	–	–	A; MF069052
	6	10	100	–	–	–	A; MF069051
Sector 38 west
	7	1000	70	E; MF084938	–	–	C; MF069071
	8	10,000	5	E; MF084935	–	–	E; MF069070
	9	10	100	–	–	–	A; MF069056
	10	10,000	90	–	–	–	E; MF069059
	11	100	60	–	–	–	D; MF069055
Punjab
Singha Devi
	12	30	67	–	–	–	A; MF069054
Kurali
	13	10,000	90	E; MF084936	E; MF095054	E; MF106203	E; MF069072
	14	10,000	70	E; MF084934	E; MF095052	–	–
	15	10,000	80	–	B; MF095053	–	B, MF069053
	16	20	100	–	–	–	B; MF069066
	17	20	50	–	–	–	B; MF069064
Jayenti
	18	10	100	–	–	–	B; MF069060
Haryana
Saketri
	19	100	10	E; MF084937	–	–	–
	20	50	60	–	–	–	B; MF069050
	21	30	30	–	–	–	A; MF069068
	22	100	40	–	–	–	A; MF069067
	23	1000	90	–	–	–	E; MF069065
	24	40	25	–	–	–	B; MF069063
	25	20	50	–	–	–	B; MF069061
	26	120	33	–	–	–	A; MF069069
	27	500	44	–	–	–	A; MF069049
	28	160	87	–	–	–	A; MF069048

PCR conditions and reaction times can be found in Additional file 1
– no amplification
TPI triosephosphate isomerase; GDH glutamate dehydrogenase; BG beta giardin; SSU small subunit; rRNA; − PCR Negative; Assemblage (GenBank Accession number) where sequence of PCR products was obtained
^aNumber of Giardia cysts used for DNA isolation
^bproportion of DAPI positive Giardia cysts used for DNA isolation
^cAdditional file 1; Ref. [4]
^dAdditional file 1; Ref. [3]
^eAdditional file 1; Ref. [7]
^fAdditional file 1; Ref. [1, 2]

ISSN: 1751-0147