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Table 5 Results from sequencing and analysis of positive polymerase chain reaction products. Giardia assemblages are noted with capital letter before semicolon preceding GenBank Accession number

From: Goats in the city: prevalence of Giardia duodenalis and Cryptosporidium spp. in extensively reared goats in northern India

Area Sample no No. of cysts isolated by IMSa DAPI (%)b GDHc TPId Bge SSUf
Chandigarh
Kansal 1 10 100 – – – B; MF069062
2 220 30 – – – E; MF069058
3 10 100 – – – A; MF069057
4 20 100 – – – B; MF069047
5 100 40 – – – A; MF069052
6 10 100 – – – A; MF069051
Sector 38 west
  7 1000 70 E; MF084938 – – C; MF069071
8 10,000 5 E; MF084935 – – E; MF069070
9 10 100 – – – A; MF069056
10 10,000 90 – – – E; MF069059
11 100 60 – – – D; MF069055
Punjab
 Singha Devi
  12 30 67 – – – A; MF069054
 Kurali
  13 10,000 90 E; MF084936 E; MF095054 E; MF106203 E; MF069072
14 10,000 70 E; MF084934 E; MF095052 – –
15 10,000 80 – B; MF095053 – B, MF069053
16 20 100 – – – B; MF069066
17 20 50 – – – B; MF069064
 Jayenti
  18 10 100 – – – B; MF069060
Haryana
 Saketri
  19 100 10 E; MF084937 – – –
20 50 60 – – – B; MF069050
21 30 30 – – – A; MF069068
22 100 40 – – – A; MF069067
23 1000 90 – – – E; MF069065
24 40 25 – – – B; MF069063
25 20 50 – – – B; MF069061
26 120 33 – – – A; MF069069
27 500 44 – – – A; MF069049
28 160 87 – – – A; MF069048
  1. PCR conditions and reaction times can be found in Additional file 1
  2. – no amplification
  3. TPI triosephosphate isomerase; GDH glutamate dehydrogenase; BG beta giardin; SSU small subunit; rRNA; − PCR Negative; Assemblage (GenBank Accession number) where sequence of PCR products was obtained
  4. aNumber of Giardia cysts used for DNA isolation
  5. bproportion of DAPI positive Giardia cysts used for DNA isolation
  6. cAdditional file 1; Ref. [4]
  7. dAdditional file 1; Ref. [3]
  8. eAdditional file 1; Ref. [7]
  9. fAdditional file 1; Ref. [1, 2]
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Acta Veterinaria Scandinavica

ISSN: 1751-0147