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Table 2 Reduction of Scrapie activity by intact and extracted yeasts and their corresponding protease activity

From: Screening of intact yeasts and cell extracts to reduce Scrapie prions during biotransformation of food waste

Yeast species

Scrapie reductiona and highest detectionb

Protease activityc

Intact yeast

Extracted yeast

Intact yeast

Extracted yeast

Aureobasidium pullulans

0/4 10−4.1

0/2 10−3.3

18.6 (4.9)

13.6 (16.7)

Blastobotrys adeninivorans

0/4 10−3.9

1/2 10−3.0

16.2 (3.8)

144.7 (60.1)

Cryptococcus laurentii

3/4 10−3.3

2/2 10−2.8

33.4 (48.9)

286.5 (6.6)

Debaryomyces hansenii

0/4 10−4.8

2/2 10−3.0

42.3 (11.0)

61.7 (41.8)

Diutina catenulata

2/4 10−3.4

0/2 10−3.8

16.7 (2.8)

22.5 (1.7)

Holtermanniella takashimae

0/4 10−4.1

0/2 10−3.5

19.8 (0.6)

19.7 (0.1)

Kluyveromyces marxianus

0/4 10−3.6

0/2 10−3.8

39.8 (46.3)

48.7 (1.1)

Naganishia cerealis

0/4 10−4.3

0/2 10−4.0

7.1 (2.1)

43.4 (63.0)

Saccharomyces cerevisiae

0/4 10−3.5

0/2 10−4.0

18.6 (2.3)

23.6 (2.6)

Wickerhamomyces anomalus

3/4 10−3.1

0/2 10−3.5

12.9 (3.1)

21.5 (0.1)

  1. aNumber of positive tests that showed 0.5–1.0 log10 reduction in Scrapie after incubation compared with a control
  2. bMean dilution of highest positive detection of Scrapie after immunoblotting
  3. cAnalysed by the fluorescence detection of trypsin (ng) from casein substrate after 60 min incubation at 37 °C