Table 2 PCRs targeting four different gene regions were used to amplify parapoxvirus-specific DNA from tissue samples obtained from 16 clinical cases of contagious ecthyma in five species of Alaskan ruminants (2001–2012)

Putative viral envelope antigen

B2L

PPP-1 → 5′-gtc gtc cac gag cag ct-3′

PPP-4 → 5′-tac gtg gga agc gcc tcg ct-3′

[23,24,25]

Granulocyte–macrophage-colony-stimulating factor (GM-CSF)/interleukin-2 inhibition factor

GIF

GIF 5 → 5′-gct cta gga aag atg gcg tg-3′

GIF 6 → 5′-gta ctc ctg gct gaa gag cg-3′

[26, 27]

Viral interleukin 10 orthologue

vIL-10

vIL-10-3 → 5′-atg cta ctc aca cag tcg ctc c-3′

vIL-10-4 → 5′-tat gtc gaa ctc gct cat ggc c-3′

[25, 28, 29]

A-type inclusion

ATI

ATIF → 5′-agc cac agc act ttc gta cat-3′

ATIR → 5′-cgc gtt tgt gtt cgt tgg at-3′)