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Table 4 Results of serological investigations, analyses in faeces (qPCR Lawsonia intracellularis and DM) as well as counts of L. intracellularis and concentrations of short chain fatty acids in caecal content and cecal crypt depth in groups of finishing boars (n = 10/treatment) fed diets with different compositions

From: Experimental studies on effects of diet on Lawsonia intracellularis infections in fattening boars in a natural infection model

Item Time Treatment
FP Mean SD CM Mean SD CORN Mean SD WHEY Mean SD RPS Mean SD
   Blood
PI values blocking ELISA TP1 (start) 31.0B 12.9 30.5B 16.9 25.5B 13.1 29.0B 14.7 32.9B 14.6
TP6 (final) 53.7A 10.3 50.1A 10.3 57.9A 4.84 57.3A 3.92 58.7A 7.15
Δ TP1 to TP6 22.7 18.9 19.6 23.3 32.4 14.2 28.3 16.2 25.8 17.6
   Faeces
log10 GE L. intracellularis (per g) TP1 (start) 2.46AB 2.64 3.58AB 2.54 3.43 2.37 2.30 3.16 2.58B 2.73
TP2 (week 1) 1.08B 2.32 1.54BC 2.48 2.52 2.68 3.75 3.51 1.90B 2.46
TP3 (week 2) 4.32A 3.16 0.59C 1.87 3.06 3.32 4.11 2.99 2.45B 3.33
TP4 (week 3) 3.64AB 3.23 5.83A 2.36 5.06 3.09 5.05 3.28 7.25A 1.44
TP5 (week 4) 4.55A 3.33 4.27A 3.35 4.98 3.42 3.11 3.37 5.46A 3.85
Ø TP2–5 (weeks 1–4) 3.40 3.23 3.03 3.25 3.78 3.19 3.95 3.23 4.08 3.59
∆ (Ø TP2–5−TP1) 0.93 3.14 − 0.57 3.30 0.38 2.65 1.68 3.58 1.51 3.80
DM content (g/kg) TP1 (start) 237 78.9 247 24.7 254 28.8 248 84.7 240 29.1
TP2 (week 1) 273a 40.9 248bc 10.1 266ab 24.8 241c 19.9 238c 32.1
TP3 (week 2) 259 84.7 238 18.0 257 97.7 245 16.7 214 18.1
TP4 (week 3) 274a 49.2 249ab 24.0 246ab 17.9 241ab 16.8 226b 55.4
TP5 (week 4) 268a 27.4 245ab 17.7 263a 24.6 245ab 17.5 222b 25.9
Ø TP2–5 (weeks 1–4) 269a 53.0 245bc 17.9 258ab 51.16 243bc 17.2 225c 35.4
   Caecal content or rather caecal wall
log10 GE L. intracellularis (per g) TP6 4.34ab 3.83 5.46a 3.03 5.16ab 3.63 1.57b 3.32 5.82a 3.51
Starch g/kg DM) TP6 41.4b 7.97 164a 35.4 136a 40.6 85.1b 22.4 179a 110
Acetate (mmol/kg FM) TP6 148 6.64 145 8.07 131 14.4 143 6.90 136 27.1
Propionate (mmol/kg FM) TP6 50.5 3.98 53.8 5.82 51.0 3.03 49.4 2.80 48.6 9.28
Butyrate (mmol/kg FM) TP6 20.3b 3.32 24.8b 3.83 21.2b 8.38 24.4b 6.09 33.7a 9.53
Crypt depth caecum (μm) TP6 482b 57.5 473b 50.0 499b 106 475b 66.0 570a 189
  1. Upper case letters (A, B) signify differences in columns (vertical) on the level of a specific feeding group between TPs (between TP1 and TP6 for “PI values blocking ELISA”; between TP1–5 for “log10 GE L. intracellularis”, between TP1–5 for “DM content”) depending on time point at P < 0.05
  2. Lower case letters (a, b, c) signify differences in a row (horizontal) between feeding groups on parameter and TP-level at P < 0.05
  3. FP fine pelleted diet, CM coarse meal diet, CORN meal diet with 22% cracked corn, WHEY meal diet with 16.9% dried whey, RPS meal diet with 30% raw potato starch, Ø abbreviation for mean value, TP1 time point 1 (start); TP2 (week 1), TP3 (week 2), TP4 (week 3), TP5 (week 4), TP6 (slaughter), PI percent inhibition, cut-off values for the blocking ELISA test with a cut-off value of PI 30, GE genome equivalents per gramme faeces, 30 days later