Fig. 1

SDS-PAGE and Western blot analysis of a serum sample (left) and milk sample (right), as indicated. The serum sample was diluted 1:1600 and the milk sample was diluted 1:40. In both cases, the purified standard mink IgG preparation was also included. All samples were electrophoresed under reducing conditions on 12% NuPAGE Bis–Tris gel as described in “Methods” section. For Western blotting (WB), the blot was developed with polyclonal goat anti-ferret IgG, followed by alkaline phosphatase-conjugated rabbit anti-goat antibody (see “Methods” section). SDS-PAGE: Lane 1: molecular weight marker; Lane 2: serum/milk sample; Lane 3: purified standard mink IgG. WB: Lane 4: molecular weight marker; Lane 5: serum/milk sample; Lane 6: purified standard mink IgG. The positions of the molecular weight marker proteins (20–100 kDa) are indicated to the left of the gels and blots