Fig. 1
From: Determination of the expression of three fimbrial subunit proteins in cultured Trueperella pyogenes
Analysis of the molecular weight and immunoreactivity of the rFim A, rFim C, and rFim E proteins by SDS-PAGE and western blot assays. a Purified rFim A, rFim C, and rFim E were analyzed via SDS-PAGE. The predicted molecular weight of rFim A, rFim C, and rFim E was 58 kDa, 58 kDa, and 72 kDa, respectively. b Immunoreactivity of rFim A, rFim C, and rFim E to rabbit anti-T. pyogenes serum. The serum was diluted 1:10,000. c Determination of the existence and the amount of rFim A, rFim C, and rFim E on the NC membrane via immunoblot assay using anti-histidine tag monoclonal antibody. The antibody was diluted 1:5000