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Table 1 Primers used for gene cloning and plasmid construction

From: Determination of the expression of three fimbrial subunit proteins in cultured Trueperella pyogenes

Names of the primers

Nucleotide (5′ to 3′)

Restrict sites

fimA forward

CGAGCTCATGGGTGCACTGGTGACGGC

Sac I

fimA backward

CCCAAGCTTTTAGGCATTACGACGACGAGCGAC

Hind III

fimC forward

CGCGGATCCGTGTTTACGGTGGTAACC

EcoR I

fimC backward

CCCAAGCTTTTAGCTACGACGACGAGCGTG

Hind III

fimE forward

CGAGCTCATGAAGCGAAACAAGTTACGTGC

Sac I

fimE backward

CCCAAGCTTTTAGACGTCCTTGCGTCGGTT

Hind III

  1. Restriction enzyme recognition sites are underlined in each sequence and listed in the right column
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Acta Veterinaria Scandinavica

ISSN: 1751-0147