Fig. 4

Genome editing systems are used to introduce double-strand breaks into DNA, which allow for gene correction, deletion or addition ZFNs, TALENs and CRISPR/Cas9 are versatile nuclease-based platforms of genome editing technology. ZFNs and TALENs consist of DNA-binding domain, which is engineered to recognize specific sequences and nuclease domain—FokI—responsible for DNA cleavage. CRISPR/Cas9 is based on RNA-guided DNA recognition complex which interacts with Cas9 nuclease catalyzing site-specific breaks in DNA. Genome editing technologies can be used for the immunotherapy purposes to enhance T cells properties