Fig. 2
From: Neuropathological findings suggestive for a stroke in an alpaca (Vicugna pacos)
Focal brain lesion in an alpaca (Vicugna pacos): Fluorescence labeling to characterize intralesional microglia/macrophages and astroglia. a–c Triple fluorescence staining of microglia/macrophages (Iba/STL), vessels (STL) and astrocytes (GFAP). Red fluorescent Cy3-immunolabeling of Iba clearly demonstrates ameboid microglia/macrophages (a) surrounding a large vessel visualized by Cy5-marked binding sites for STL (b, color-coded in blue). The merged staining patterns (c) also display an enhanced GFAP-immunoreactivity (Cy2, green) at the vascular border. d–f Simultaneous staining of astroglial endfeet (AQP4) and astrocytic intermediate filaments (GFAP) combined with vessel detection (STL) in severely affected tissue. In parallel, the concomitantly enhanced immunoreactivities for AQP4 (d, Cy3) and GFAP (e, Cy2) surround a STL-stained large vessel (Cy5, color-coded in blue) as shown in (f). Bars: c (also valid for a and b): 100 µm, f (also valid for d and e): 150 µm