Fig. 1
From: The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa
Experimental scheme. All semen samples (ejaculated sperm and epididymal sperm) underwent a quality assessment. Ejaculated and sperm from epididymal tails were equally treated and examined. Each sperm sample was aliquoted (n = 2 aliquots per group, 200 µL each), diluted and incubated with probucol (experimental sample) or without probucol (control sample). Remaining raw sperm samples were centrifuged and the seminal plasma/cauda epididymal fluids stored for cholesterol measurement. After incubation, experimental and control samples were centrifuged and the pellet further processed for CTC assay. The supernatant was stored for cholesterol measurement