Low occurrence of extended-spectrum cephalosporinase producing Enterobacteriaceae and no detection of methicillin-resistant coagulase-positive staphylococci in healthy dogs in Sweden

Table 1 Genotypic and phenotypic characteristics in three Escherichia coli isolated from Swedish dogs from May 2017 to May 2018, and from a screening study conducted in 2012

Year	Isolate	Genes encoding ESBL	MLST	Serotype	Plasmid replicon types^a	Genes encoding antibiotic resistance	Antibiotic resistance	MIC for Az^b
2012	CH79ctx	bla_CMY-2	ST38	O7:H18	colMG828, B/O/K/Z, FIB FII, p0111	mdfA	Am, Cx, Cm	4
2017	ACS2	bla_CTX-M-1	ST4496	O8:H28	FIA, HI1A, HI1B, Q1	aac(3)-IIb, aadA2, aph(3″)-Ib, aph(6)-Ib, catA1, dfrA12, mdfA, sul1, sul2, tetA, mphA	Am, Cx, Cm, Chl, Gm, Su, Tm, Tc	8
	ACS5	bla_CTX-M-55	ST354	O1:H34	col156, FIA, FIB, FII, Q1	bla_TEM-1B, aac(3)-IId, aadA5, ant(3″)-Ia, aph(3″)-Ib, aph(6)-Ib, catA1, dfrA17, mdfA, mphA, sul1, sul2, tetA, tetD	Am, Cx, Cm, Chl, Ci, Gm, Nal, Su, Tm, Tc	16
	ACS6	bla_CTX-M-27	ST131	O25:H4	col156, col8282, FIA, FIB, FII	aadA5, ant(3″)-Ia, aph(3″)-Ib, aph(6)-Id, dfrA17, mdfA, mphA, sul1, sul2, tetA	Am, Cx, Cm, Ci, Nal, Su, Tm, Tc	32

All isolates were tested for susceptibility against ampicillin (Am), azithromycin (Az), cefotaxime (Cx), ceftazidime (Cm), chloramphenicol (Chl), ciprofloxacin (Ci), colistin (Co), gentamicin (Gm), meropenem (Me), nalidixic acid (Nal), kanamycin (Km), sulfamethoxazole (Su), trimethoprim (Tm), tigecycline (Tg) and tetracycline (Tc). Isolates were defined as resistant if minimum inhibitory concentration (MIC) was above the epidemiological cut-off values (ECOFFs) defined by EUCAST
^aPlasmidfinder (https://cge.cbs.dtu.dk/) cannot differentiate between incB, incO, incK, incZ
^bNo ECOFF defined by EUCAST

ISSN: 1751-0147