Skip to main content

Table 4 Multivariate analysis for semen quality characteristics of fresh and frozen-thawed semen and elements Fe, Se and Zn measured in blood serum on the day of collection

From: Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

Variables Elements in blood serum (mg/L)
Fe Se Zn
Semen characteristics (%) β (95% CI) P β (95% CI) P β (95% CI) P
Fresh semen
 Motilitya 80,738 (−39,156 to 2,00,632) 0.1781 8,02,538 (4,61,390–1,143,685) 0.0001 94,064 (39,659–1,48,469) 0.0015
 Progressive motilitya 82,652 (−20,842 to 1,86,147) 0.1127 6,49,692 (3,55,208–9,44,176) 0.0001 44,795 (−2169 to 91,758) 0.0607
 Viabilitya 1,23,403 (−7048 to 2,53,853) 0.0627 7,82,244 (4,11,058–1,153,430) 0.0002 47,977 (−11,218 to 1,07,173) 0.1077
 Normal morphologya −5386 (−1,21,992 to 1,11,220) 0.9251 6,08,177 (2,76,385–9,39,969) 0.0009 1,03,614 (50,701–1,56,528) 0.0004
 Acrosome defectsb −0.6213 (−1.3876 to 0.1451) 0.1076 −0.7251 (−2.9057 to 1.4555) 0.5003 −0.4135 (−0.7613 to −0.0658) 0.0216
 Normal tail membrane integritya −85,817 (−2,25,635 to 54,001) 0.2183 5,05,314 (1,07,474–9,03,153) 0.0148 70,115 (6669–1,33,561) 0.0316
 DNA fragmentation −3.5201 (−6.4732 to −0.5670) 0.0213 −6.2227 (−14.6255 to 2.1802) 0.1400 −1.0848 (−2.4249 to 0.2552) 0.1081
Frozen-thawed semen
 Motilitya 38,919 (−75,091 to 1,52,930) 0.4891 7,81,556 (4,57,150–1,105,962) 0.0000 71,343 (19,608–123,078) 0.0088
 Progressive motilitya 4328 (−65,223 to 73,878) 0.8992 2,33,317 (35,418–4,31,216) 0.0226 52,522 (20,962–84,083) 0.0021
 Viabilitya 67,129 (−72,219 to 2,06,477) 0.3312 6,34,447 (2,37,944–1,030,950) 0.0029 95,076 (31,843–1,58,309) 0.0047
 Normal morphologya −59,182 (−1,73,925 to 55,561) 0.2988 5,48,513 (2,22,021–8,75,005) 0.0019 91,924 (39,856–1,43,992) 0.0012
 Acrosome defectsb 0.0237 (−0.4278 to 0.4752) 0.9149 −1.2739 (−2.5585 to 0.0107) 0.0518 −0.1664 (−0.3713 to 0.0385) 0.1070
 Normal tail membrane integritya −19,935 (−1,43,509 to 1,03,638) 0.7428 8,14,528 (4,62,911–1,166,146) 0.0001 47,923 (−8152 to 1,03,998) 0.0907
 DNA fragmentation −1.5635 (−5.6921 to 2.5652) 0.4434 −13.9299 (−25.6775 to −2.1822) 0.0219 −2.4675 (−4.3410 to −0.5940) 0.0118
  1. aCubic transformation
  2. bLog transformation