From: Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance
Variables | Elements in blood serum (mg/L) | |||||
---|---|---|---|---|---|---|
Fe | Se | Zn | ||||
Semen characteristics (%) | β (95% CI) | P | β (95% CI) | P | β (95% CI) | P |
Fresh semen | ||||||
Motilitya | 80,738 (−39,156 to 2,00,632) | 0.1781 | 8,02,538 (4,61,390–1,143,685) | 0.0001 | 94,064 (39,659–1,48,469) | 0.0015 |
Progressive motilitya | 82,652 (−20,842 to 1,86,147) | 0.1127 | 6,49,692 (3,55,208–9,44,176) | 0.0001 | 44,795 (−2169 to 91,758) | 0.0607 |
Viabilitya | 1,23,403 (−7048 to 2,53,853) | 0.0627 | 7,82,244 (4,11,058–1,153,430) | 0.0002 | 47,977 (−11,218 to 1,07,173) | 0.1077 |
Normal morphologya | −5386 (−1,21,992 to 1,11,220) | 0.9251 | 6,08,177 (2,76,385–9,39,969) | 0.0009 | 1,03,614 (50,701–1,56,528) | 0.0004 |
Acrosome defectsb | −0.6213 (−1.3876 to 0.1451) | 0.1076 | −0.7251 (−2.9057 to 1.4555) | 0.5003 | −0.4135 (−0.7613 to −0.0658) | 0.0216 |
Normal tail membrane integritya | −85,817 (−2,25,635 to 54,001) | 0.2183 | 5,05,314 (1,07,474–9,03,153) | 0.0148 | 70,115 (6669–1,33,561) | 0.0316 |
DNA fragmentation | −3.5201 (−6.4732 to −0.5670) | 0.0213 | −6.2227 (−14.6255 to 2.1802) | 0.1400 | −1.0848 (−2.4249 to 0.2552) | 0.1081 |
Frozen-thawed semen | ||||||
Motilitya | 38,919 (−75,091 to 1,52,930) | 0.4891 | 7,81,556 (4,57,150–1,105,962) | 0.0000 | 71,343 (19,608–123,078) | 0.0088 |
Progressive motilitya | 4328 (−65,223 to 73,878) | 0.8992 | 2,33,317 (35,418–4,31,216) | 0.0226 | 52,522 (20,962–84,083) | 0.0021 |
Viabilitya | 67,129 (−72,219 to 2,06,477) | 0.3312 | 6,34,447 (2,37,944–1,030,950) | 0.0029 | 95,076 (31,843–1,58,309) | 0.0047 |
Normal morphologya | −59,182 (−1,73,925 to 55,561) | 0.2988 | 5,48,513 (2,22,021–8,75,005) | 0.0019 | 91,924 (39,856–1,43,992) | 0.0012 |
Acrosome defectsb | 0.0237 (−0.4278 to 0.4752) | 0.9149 | −1.2739 (−2.5585 to 0.0107) | 0.0518 | −0.1664 (−0.3713 to 0.0385) | 0.1070 |
Normal tail membrane integritya | −19,935 (−1,43,509 to 1,03,638) | 0.7428 | 8,14,528 (4,62,911–1,166,146) | 0.0001 | 47,923 (−8152 to 1,03,998) | 0.0907 |
DNA fragmentation | −1.5635 (−5.6921 to 2.5652) | 0.4434 | −13.9299 (−25.6775 to −2.1822) | 0.0219 | −2.4675 (−4.3410 to −0.5940) | 0.0118 |