Serum biochemistry and haematology in wild and captive bearded seals (Erignathus barbatus) from Svalbard, Norway

Table 1 Analytical methods used for serum biochemistry analyses of bearded seals (Erignathus barbatus) from Svalbard, Norway

Analyte^a	Method/principle	Manufacturer^b	References
AST	Modified IFCC^c	Siemens	[4]
ALT	Modified IFCC	Siemens	[4]
ALP	Modified IFCC	Siemens	[4]
CK	N-acetylcysteine act., DGKC	Siemens	[4]
LDH	Enzymatic/Tris/NAD	Siemens	[4]
Amylase	Ethylidene blocked-pNPG7	Siemens	[4]
Lipase	Kinetic colorimetric	Sera-Pak	[5]
Albumin	Serum protein electrophoresis	Beckman	—
Bilirubin (total)	Diazo/caffeine	Siemens	[4]
Calcium	CPC	Siemens	[6]
Chloride	Ion-selective electrode, diluted	Siemens	[4]
Cholesterol	Enzymatic	Siemens	[7]
Cortisol	Solid-phase, chemiluminescent, competitive immunoassay	DPC	[8]
Creatinine	Jaffe, alkaline picrate, kinetic	Siemens	[9]
NEFA	ACS-ACOD-MEHA	Wako	[10]
Glucose	Hexokinase	Siemens	[11]
Magnesium	Xylidyl blue	Siemens	[12]
Phosphorus (inorg.)	Phosphomolybdate/ultraviolet	Siemens	[13]
Potassium	Ion-selective electrode, diluted	Siemens	[4]
Protein (total)	Biuret	Siemens	[4]
Sodium	Ion-selective electrode, diluted	Siemens	[4]
Thyroxine	Solid-phase, chemiluminescent, competitive immunoassay	Siemens	[14]
Triglycerides	GPO, trinder without serum blank	Siemens	[15]
Urea	Urease with GLDH	Siemens	[16]

^aAST Aspartate aminotransferase, ALT Alanine aminotransferase, ALP Alkaline phosphatase, CK Creatine kinase, LDH Lactate dehydrogenase, NEFA Non-esterified fatty acids
^bSiemens Healthcare Diagnostics (including Sera-Pak), Tarrytown, NY, USA; Beckman Coulter, Inc, Fullerton, CA, USA; DPC: Diagnostic Products Corporation, Los Angeles, CA, USA; Wako Chemicals GmbH, Neuss, Germany
^cIFCC International Federation of Clinical Chemistry, pNPG7 p-nitro-phenylmalto-heptaoside, NAC N-acetylcysteine, DGKC Deutsche Gesellschaft für Klinische Chemie, CPC o-cresolphtalein complexone, ACS-ACOD-MEHA Acyl-CoA-Synthetase-Ascorbate oxidase- 3-Methyl-N-Ethyl-N-(ß-hydroxyethyl)aniline, GPO glycerol-3-phosphate oxidase, GLDH glutamate dehydrogenase

ISSN: 1751-0147