Fig. 6From: Early immunohistochemical detection of pulmonary micrometastases in dogs with osteosarcomaa Section of lung tissue from a dog (case 3) showing immunolabeling (red staining) with tumour protein-3 (TP-3) of the respiratory bronchiolar epithelium. An asymmetrical staining pattern can be seen, with a stronger reaction towards the ciliated brush border. b Section of the same tissue as in a, but without TP-3 antibody (negative control). Snap frozen tissue, immunoperoxidase stain, TP-3, AEC chromogen and haematoxylin counterstain, 100 × magnificationBack to article page