Centrifugal force assessment in ram sperm: identifying species-specific impact

Table 1 Effect on ram sperm kinetic parameters induced by three centrifugal forces

Parameter	Control	600×g	3000×g	6000×g
*VCL (µm/s)*
0 h	296.6 ± 17.9^ab	307.9 ± 16.0^a	289.0 ± 16.7^ab	277.9 ± 8.9^b
2 h	318.3 ± 14.8^a	307.5 ± 14.0^a	284.5 ± 13.9^b	274.1 ± 10.3^b
*VSL (µm/s)*
0 h	89.7 ± 4.6^ab	93.9 ± 4.5^a	87.7 ± 5.5^ab	86.8 ± 4.8^b
2 h	80.4 ± 3.0	83.7 ± 3.4	81.4 ± 4.5	81.6 ± 5.1
*VAP (µm/s)*
0 h	148.7 ± 6.0^ab	153.5 ± 5.5^a	142.9 ± 6.7^ab	141.6 ± 3.3^b
2 h	152.2 ± 5.1^a	145.9 ± 4.9^a	135.7 ± 4.7^b	134.0 ± 3.2^b
*LIN (%)*
0 h	31.8 ± 2.1	31.6 ± 1.9	30.7 ± 1.9	31.8 ± 1.8
2 h	26.6 ± 1.4^a	28.0 ± 1.5^ab	29.2 ± 1.6^b	30.1 ± 1.8^b
*STR (%)*
0 h	59.9 ± 3.2	60.1 ± 2.6	59.2 ± 3.0	59.7 ± 3.0
2 h	52.7 ± 2.1^a	56.3 ± 2.6^ab	57.7 ± 2.7^bc	58.8 ± 3.2^bc
*WOB (%)*
0 h	52.0 ± 1.3	51.2 ± 1.2	50.3 ± 1.1	51.9 ± 1.0
2 h	49.4 ± 0.8^ab	48.6 ± 0.7^a	49.0 ± 0.9^ab	50.1 ± 1.0^b
*ALH (µm)*
0 h	4.1 ± 0.2^ab	4.2 ± 0.2^a	3.9 ± 0.2^ab	3.8 ± 0.1^b
2 h	4.3 ± 0.2^a	4.2 ± 0.2^a	3.9 ± 0.2^b	3.7 ± 0.1^b
*BCF (Hz)*
0 h	24.4 ± 1.4	25.9 ± 1.6	26.2 ± 1.6	26.5 ± 1.4
2 h	22.5 ± 0.9^a	22.9 ± 0.7^ab	24.0 ± 0.8^bc	24.8 ± 0.9^c

The same 11 males were analyzed in each experimental group (600×g, 3000×g, and 6000×g for 10 min at room temperature) at 0 h and 2 h post-incubation at 37 °C. Different lowercase superscripts letters (a, b, c) indicate significant differences (P ≤ 0.05) for each parameter and assessment point among the centrifugal forces
VCL, curvilinear velocity (µm/s); VSL, straight line velocity (µm/s); VAP, path velocity (µm/s); LIN, linearity (%); STR, straightness (%); WOB, wobble (%); ALH, head lateral amplitude (µm); BCF, beat frequency (Hz)

ISSN: 1751-0147