Table 2 Enterococcus faecium resistance gene primer sequence and product length
Target genes | Primer sequence (5′–3′) | Product size (bp) |
|---|---|---|
blaTEM | CCCCGAAGAACGTTTTC ATCAGCAATAAACCAGC | 516 |
blaSHV | TCAGCGAAAAACACCTTG TCCCGCAGATAAATCACCA | 475 |
blaCTX-M | TCAGCGAAAAACACCTTG GATATCGTTGGTGGTGCCAT | 543 |
optrA | AGGTGGTCAGCGAACTAA ATCAACTGTTCCCATTCA | 1395 |
cfr | TGAAGTATAAAGCAGGTTGGGAGTCA ACCATATAATTGACCACAAGCAGC | 746 |
poxtA | GGTGGATTTACCGACACCGT GACCAGTGGAAATGCCCGTA | 943 |
aac(6′)-aph(2′′) | GAGCAATAAGGGCATACCAAAAATC CCGTGCATTTGTCTTAAAAAACTGG | 505 |
aph(2′′)-Ib | TATGGATTCATGGTTAACTTGGACGCT GAG ATTAAGCTTCCTGCTAAAATATAAACA TCTCTGCT | 906 |
aph(2′′)-Ic | GAAGTGATGGAAATCCCTTCGTG GCTCTAACCCTTCAGAAATCCAGTC | 627 |
aph(2′′)-Id | GGTGGTTTTTACAGGAATGCCATC CCCTCTTCATACCAATCCATATAACC | 642 |
vanA | GGGAAAACGACAATTGC GTACAATGCGGCCGTTA | 732 |
vanB | ATGGGAAGCCGATAGTC GATTTCGTTCCTCGACC | 635 |
ermA | TCTAAAAAGCATGTAAAAGAA CGATACTTTTTGTAGTCCTTC | 645 |
ermB | GAAAAGGTACTCAACCAAATA AGTAACGGTACTTAAATTGTTTA | 639 |