Table 1 Primers and PCR conditions. Primers for the rRNA and ITS regions [30,31,32] and for cox1 [33–34] were applied. Pre-denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 30 s, annealing at primer specific temperature for 30 s and elongation at 72 °C for a primer specific time, and finally a post elongation at 72 °C for 5 min. For the mtDNA PCR a touchdown procedure was applied: annealing at 53 °C 2 cycles, 51 °C 2 cycles, 50 °C 2 cycles, 49 °C 3 cycles, 48 °C 3 cycles, 47 °C 3 cycles, 46 °C 35 cycles

From: Transversotrema hafniensis n. sp. infection in Poecilia reticulata by cercariae released from Melanoides tuberculata in Denmark