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Prevalence of Teat Apex Colonizations by S. Chromogenes in Young and Primigravid Dairy Heifers

  • 1, 2,
  • 1, 2,
  • 1,
  • 1,
  • 1 and
  • 1
Acta Veterinaria Scandinavica200344 (Suppl 1) :P90

https://doi.org/10.1186/1751-0147-44-S1-P90

  • Published:

Keywords

  • Agar
  • Agar Plate
  • Bacterial Growth
  • Veterinary Medicine
  • Blood Agar

Little attention has been paid to intramammary infections (IMI) in dairy heifers as their non-lactating udders have traditionally been considered as uninfected. However, high prevalences of IMI in non-lactating and freshly calved heifers, mainly caused by Non-aureus staphylococci (NAS), were reported [1]. Prevalence of teat apex and teat skin colonizations is also high. They are mainly caused by the same species [3]. The aim of this study was to examine the prevalence of teat apex colonization with Staphylococcus chromogenes, one of the most prevalent NAS, in young and primigravid dairy heifers, and the effect of age on this prevalence.

In a cross-sectional study, teat ends (n = 492) were sampled from in total 123 heifers, on eight dairy herds. The age of the heifers varied from 8 to 34 months. The heifers were immobilized and the teat apexes were cleansed with a sterile cloth, sampled with a swab (Cultiplast® and put on to a transport medium. All swabs were streaked on a blood agar plate and incubated aerobically at 37°C. Bacterial growth was recorded after 24 and 48 hours of incubation. Identification was performed as described by [2]. A teat apex was classified as infected with S. chromogenes when at least 8 colony forming units were isolated. Prevalences were calculated as the percentage of heifers with at least one infected teat apex and as the percentage of infected teat apexes. The mean number of infected teat apexes per heifer was also calculated. Logistic regression was used to analyze the effect of age of the heifers on prevalence of S. chromogenes. Odds Ratio's (OR) per age group, using the youngest age group as reference, were calculated (95% Confidence Intervals-CI)(SPSS 10.0).

The mean age of the heifers at the moment of sampling was 18.7 months. Overall, 20.3% of the heifers had S. chromogenes infected teat apexes, while 9.7% of all teat apexes were infected with S. chromogenes. There was a significant influence of age class on the prevalence of infected heifers (P < 0.05). Prevalences of infected heifers (with OR), prevalences of infected teat apexes per age class, and mean number of infected teat apexes per heifer are presented in Table 1.
Table 1

Prevalences of S. chromogenes infected heifers and S. chromogene s infected teat apexes per age class and mean number of infected teat apexes per heifer

Age class

N heifers

Prevalence % of heifers

OR 95% CI

Prevalence % of apexes

Mean infected number of infected teat apexes per heifer

8 – 12 months

22

91%

 

45%

2

gt12 – 20 months

52

77%

08 01 – 49

34%

175

gt20 – 28 months

37

351%

54 11 – 269

162%

185

gt28 – 34 months

12

500%

100 16 – 631

271%

216

This study shows that heifers older than 20 months of age run a higher risk of having S. chromogenes infected teat apexes than younger ones Further longitudinal studies will reveal whether IMI post partum can be predicted by S. chromogenes teat apex colonizations and whether there is a quantitative effect If an association exists possible risk factors for teat apex colonization can be identified and can be used to reduce the high prevalence of IMI in freshly calved dairy heifers

Authors’ Affiliations

(1)
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
(2)
Veterinary Epidemiology Unit, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium

References

  1. Boddie RL, Nickerson SC, Owens WE, Watts JL: Udder microflora in nonlactating heifers. Agri-Practice. 1987, 8: 22-25.Google Scholar
  2. Devriese LA, Laevens H, Haesebrouck F, Hommez J: A simple identification scheme for coagulase negative Staphylococci from bovine mastitis. Res Vet Sci. 1994, 57: 240-244. 10.1016/0034-5288(94)90064-7.View ArticlePubMedGoogle Scholar
  3. White DG, Harmon RJ, Matos JE, Langlois BE: Isolation and identification of coagulase-negative Staphylococcus species from bovine body sites and streak canals of nulliparous heifers. J Dairy Sci. 1989, 72: 1886-1892. 10.3168/jds.S0022-0302(89)79307-3.View ArticlePubMedGoogle Scholar

Copyright

© The Author(s); licensee BioMed Central Ltd. 2003

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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