- Original article
- Open Access
Uptake of Colostral Immunoglobulins by the Compromised Newborn Farm Animal
- P.T. Sangild1
© The Author(s); licensee BioMed Central Ltd. 2003
Published: 31 March 2003
Neonatal mortality is very high in farm animals (~10%) and disease resistance is greatly influenced by an adequate passive immunisation just after birth. In piglets, foals, calves and lambs, the intestinal absorption of immunoglobulins from their mother's colostrum occurs mainly by a non-specific endocytosis of macromolecules, but the details of the absorption process, and the mechanisms regulating its cessation after 1–2 days of colostrum exposure, remain poorly understood. In both normal and 'compromised' (premature, growth-retarded, hypoxic, lethargic) newborn farm animals, the intestinal capacity to absorb macromolecules is influenced by both diet- and animal-related factors. Thus, macromolecule uptake is severely reduced in response to premature birth and when macromolecules are to be absorbed from diets other than species-specific colostrum. On the other hand, fetal growth retardation, in vitro embryo production, or a stressful birth process are unlikely to reduce the ability of the intestine to absorb immunoglobulins from colostrum. More knowledge about the diet- and animal-related factors affecting intestinal immunoglobulin uptake will improve the clinical care of 'compromised' newborn farm animals. The present text gives a brief introduction to the process of intestinal immunoglobulin absorption in large farm animals and describe some recent results from the author's own studies in pigs, calves and lambs.
Intestinal macromolecule uptake and gut closure in farm animals
A large neonatal mortality in farm animals indicates that the neonates in these species often fails to adapt adequately to postnatal life. Neonatal vitality often shows a positive correlation with the degree of passive immunisation [14, 84, 90], and circulating immunoglobulins, particularly immunoglobulin G (IgG), constitute a key element in the general host defence against environmental antigens. Before birth, the fetus is well protected from antigens by the protective barrier of the placenta. Just after birth, the newborn must be able to respond immunologically to a massive invasion of potentially harmful antigens and microorganisms from the surrounding environment. The innate immune defence is immature at the time of birth and the specific immune system non existent. Passive immunisation from the mother is required until the active immune system is fully developed [43, 15, 1, 72]. Humans and some other species are born with passive immunity in the form of maternal immunoglobulins transferred across the placenta before birth. In contrast, the newborns of large farm animals (calves, piglets, foals, lambs) are dependent on the intestinal transmission of immunoglobulins and other immune modulating factors present in colostrum [14, 4]. Besides immunoglobulins, colostrum contains lymphocytes, cytokines, nucleotides and various growth factors which may affect the development of the immune system postnatally [9, 10, 70].
The ability of intestinal cells to take up macromolecules, including immunoglobulins, by endocytosis and to transport these molecules intact across the epithelium into the blood stream is one of the most striking and unique characteristics of the developing intestine. In the large farm animals, this ability ceases within the first day or two after birth by a process known as "intestinal closure". In other species, intestinal closure is delayed until several weeks after birth (rat, mouse, ferret) [18, 4]. In humans, only the fetal small intestine has the characteristics required for the uptake of intact immunoglobulins .
In the pig, intestinal closure begins about 6–12 h after feeding colostrum and progresses rapidly thereafter to completion at 24–36 h [88, 89]. If animals are fasted for a period after birth [46, 47, 45] or if they are fed parenteral nutrients in stead of luminal nutrients , the period of macromolecule transfer ("the pre-closure period") is prolonged up to several days. The signals inducing closure vary among species but may involve both colostral and systemic factors as well as the maturity of the gut epithelium itself [19, 73, 4]. For instance, glucocorticoids appear to reduce the capacity for and duration of macromolecule transfer in suckling rats [21, 22] but have the opposite effect in newborn lambs  and pigs .
Until now, the predominent view in the literature has been that the intestinal macromolecule uptake capacity is a fundamental characteristic of an immature epithelium and that a major part of this ability is simply due to the fact that the epithelium is "leaky" and allows greater transport of all large molecules, not only immunoglobulins . This opinion is supported by the observation that a limited macromolecule transfer takes place across the intestine in infants, despite the fact that passive immunity is aquired in utero via the transplacental passage of immunoglobulins. Quantitatively, the absorption of intact proteins in human neonates is very low, compared with that in newborn farm animals, and it decreases with advancing fetal and postnatal age . Human infants delivered prematurely therefore have a greater ability to absorb globulins from the gut than those born at term .
As we shall see in the following, the conditions for absorption of globulins in farm animals involve much more than just the presence of a "leaky epithelium" around birth. Although the epithelium of the fetus and newborn may be more permeable for the ions (sodium, chloride) and small molecules (lactulose, monosaccharides) generally used to assess intestinal permeability this does not mean that the epithelium allows transfer of immunoglobulins. The different groups of molecules are transported by completely different mechanisms across the epithelium. In this review, we shall document that the ability of the intestinal enterocytes to absorb large amounts of immunoglobulins (and other macromolecules) by endocytosis is a highly specialized process that develops close to term and then disappears shortly after birth in response to enteral feeding. The rapid developmental changes in transport before and after birth contributes to the fact that only small deviations from normal physiological conditions at birth may reduce the intestinal capacity to absorb immunoglobulins. Impaired passive immunisation may lead to compromised disease resistance, both short-term and long-term [84, 90, 27].
Intestinal selectivity exists among the IgGs from different species [25, 39] and absorption does not appear to occur entirely by non-specific endocytosis of macromolecules. A preference to transport species-specific IgG from the gut lumen to the circulation, independently of diet, could be due to the presence of specific IgG receptors on intestinal enterocytes, similar to the Fc-receptors known to exist on the brush-border membrane of enterocytes in young rodents  and in humans during fetal life . So far an Fc-receptor has not been identified in the intestine of piglets or calves [75, 55]
The degree of passive immunisation after colostrum feeding, depends not only on the intestinal capacity to absorb large molecules, but also on the availability of colostrum and its immunoglobulin concentration. These factors are subject to a lot of individual variation and may be affected by time and mode of delivery, breed and parity of the mother. Detailed information on this can be obtained from other sources [44, 34, 86]. In the present review we shall focus exclusively on factors related to the newborn and its intestinal capacity to absorb immunoglobulins.
Diet-related factors affecting intestinal macromolecule uptake
Artificial rearing of newborn animals often requires the use of suitable alternatives to mother's colostrum, and milk substitutes must have a nutritional composition that match the biological needs of the neonate. Colostrum also contains specific bioactive factors (hormones, growth factors) required for the enterocytes to function and develop normally. A series of recent studies indicate that such nutrient-independent factors in colostrum exert distinct effects on the immature enterocyte and its ability to digest and absorb nutrients [12, 91]. Such factors will be absent in most colostrum substitutes. When artificial diets are used to nourish neonates that experience an inadequate adaptation to postnatal life (e.g. premature or growth-retarded neonates), the composition of the diet (nutrients, bioactive factors) is particularly important for optimal survival and growth.
Feeding bovine colostrum to newborn pigs, without added porcine immunoglobulins, is associated with reduced survival [50, 23, 27]. Nevertheless, survival is clearly better than in pigs fed a milk replacer completely devoid of immunoglobulins . Since the antigen specificity of immunoglobulins determines the degree to which they provide immunological protection, it is not surprising that bovine immunoglobulins cannot provide notable passive immunity against pig pathogens. Regardless, the results show that colostrum per se may contain nutrients and bioactive components that have a positive effect on gut maturation and disease resistance in a species-independent manner. This hypothesis is supported by the documented positive effects of feeding bovine colostrum to pigs with weaning-associated diarrhoea  or humans showing drug-induced intestinal disease .
Premature labour in the large farm animals is often associated with clinical complications in the pregnant mother and/or the developing fetus. It is most commonly observed as a response to a clinical or subclinical state of infection, but premature birth may also occur spontaneously, without any obvious etiology. In case of viral or bacterial infection, the associated production of toxins directly or indirectly leads to the maternal, placental and/or fetal endocrine changes required to initiate the labour process. An inflammatory response, such as that reflected by elevated production of interleukins, has been shown to play a role in many cases of preterm labour .
Intestinal endocytotic capacity in the fetus
In the studies on pig fetuses , the gradual decline in protein absorptive capacity in pigs fed colostrum occurred slower than in newborn pigs, and the intestine retained some protein absorptive capacity even after 3–4 days of exposure to colostrum in utero. The factors responsible for mediating closure in both fetal pigs and newborn pigs are known to be present in the whey fraction of colostrum. The fact that the infused volume of colostrum whey in the fetal pig studies was much less (40 ml kg-1d-1) than that normally taken up by suckling newborn pigs (400 ml kg-1 d-1), is unlikely to have affected the results, regarding intestinal closure. Volumes as small as 10 ml kg-1 are known to be effective in inducing closure at the expected time in newborn pigs . The extended pre-closure period in fetuses may be explained by a functional immaturity of the intestine in the weeks before term which prolongs the protein absorptive period. Further, the state of parenteral nutrition in utero (via the placenta) may delay intestinal closure in fetuses similar to the delayed closure observed in parenterally-fed neonatal pigs , and in newborn pigs and calves fasted for some time after birth [83, 46]. In conclusion, the results on fetuses have substantiated the view that the final maturation of the epithelium for macromolecule absorption occurs in close association with the prepartum processes leading to delivery.
Birth by cesarean section
Cesarean section, as a mode of delivery, can be used for a number of reasons, but is usually performed to protect the mother during labour (dystocia) and to prevent the fetus from suffering excessively from physical and metabolic stress (hypoxia, acidosis) during vaginal birth. When cesarean section is performed during labour ("warm cesarean section"), both the mother and fetus have already been exposed to the physical, metabolic and endocrine changes associated with normal parturition. Such exposure is absent when cesarean section is performed on term or preterm pregnant mothers not in labour. The latter mode of delivery is denoted "elective cesarean section" and is often associated with a decreased survival of the newborn, apparently due to some lack of "birth stress". This is reflected in the significantly lowered levels of plasma cortisol in newborn pigs delivered by elective cesarean section, both preterm and at normal term . This could be important for the macromolecule uptake capacity since glucocorticoid hormones are known to affect the early maturation of the intestinal epithelium in many species [29, 62].
In piglets delivered at full term the initial IgG and BSA uptake capacity is only marginally reduced in response to cesarean section, and coupled with a longer pre-closure period, the resulting IgG levels were actually higher in cesarean-delivered animals than in those born vaginally . These results provide some indirect evidence for a stimulating effect of vaginal birth on the initial macromolecule absorptive capacity in the (premature) intestine. Nevertheless, the simultaneous effect on the timing of intestinal closure makes it difficult to ascertain whether the final IgG levels will be elevated or lowered in cesarean-delivered animals. The different responses at different fetal ages at delivery may be due to developmental changes in the intestinal responsiveness to glucocorticoids. It remains unclear however, whether elective cesarean section, via the lack of cortisol, influences the intestinal immunoglobulin uptake capacity in the large farm animals. Therefore a more detailed look at the effects of cortisol (the main glucocorticoid in farm animals) on the developing intestinal epithelium is warranted.
The role of cortisol
In cesarean-delivered pigs, metyrapone treatment seems to be associated with a decreased absorptive capacity of the intestinal epithelium [56, 61], while the effect in cesarean-delivered lambs is due to both a reduction in the absorptive capacity and in the time available for absorption . The effect of depressed cortisol may depend on the time of delivery. In premature lambs, there are effects both on the initial rate of protein uptake and on the timing of intestinal closure. In term lambs, the effect on closure is dominant . It is possible that the effects of cortisol on the endocytotic capacity of intestinal cells disappear shortly before term when intestinal function is relatively mature, partly mediated by the normal surge in plasma cortisol. The effect may also disappear earlier in the lamb than in the pig as the prenatal rise in cortisol occurs more gradually in the lamb compared with the pig [71, 24].
Treatment of newborn pigs [56, 61], calves [42, 76] or foals  with ACTH does not increase immunoglobulin absorption in these species. A glucocorticoid related maturation of the absorptive capacity may have occurred already before birth in these species and prevented an additional increase in glucocorticoid exposure from having any effect. This hypothesis is consistent with the fact that ACTH treatment induces a slight increase in the immunoglobulin absorptive capacity in premature cesarean-delivered lambs (92% gestation) whereas there is no effect when lambs are delivered closer to term (95% gestation) . In preterm lambs, a single peak in plasma cortisol (following an ACTH injection) has the same stimulating effect as continuous treatment with cortisol acetate . This suggests that immature intestinal enterocytes only require a short-term exposure to high glucocorticoid levels to express their full endocytotic potential.
Treatment of pregnant cows with a long-acting glucocorticoid does not prevent the reduction in immunoglobulin absorption in calves caused by premature birth [2, 32]. This can be explained by an immaturity of the intestine as immunoglobulin absorption is normal in calves following glucocorticoid induced birth close to normal term . Although premature calves born after glucocorticoid induction may absorb less immunoglobulin than normal newborn calves, they do absorb significantly higher amounts than premature calves delivered by elective cesarean section . The effects of glucocorticoids on intact protein absorption thus depend on the maturity of the intestinal cells at treatment, and the period of maximal glucocorticoid responsiveness before and after birth varies between the pig, lamb and calf.
Dystocia causes a reduction in neonatal viability in farm animals [54, 6] and in some studies, the increased morbidity has been associated with a reduced IgG absorption [13, 58]. In particular, studies in calves have shown that dystocia-induced respiratory acidosis and hypercapnia are followed by decreased absorption of IgG . This inhibition of uptake may occur both at the level of initial endocytotic capacity in intestinal cells and by an earlier induction of intestinal closure. Nevertheless, it remains unclear whether dystocia alone can influence IgG absorption  and to which extent this is directly or indirectly related to postnatal acidosis and hypercapnia . In this context, it is important also to consider the circulating cortisol levels which might influence the absorptive capacity (see above). Thus, elevated cortisol levels in response to excessive physical and metabolic stress around the time of birth could mature the immature enterocytes and cause enhanced (rather than impaired) absorption. The circulating cortisol level has been found to be elevated in dystocial and acidotic neonatal calves in some studies  while in others the level has been lowered . In a recent study , we found no correlation between the cortisol levels and the extent of birth difficulties for newborn calves, but cortisol levels during the first 2 h after birth correlated negatively with peak plasma IgG concentration. Hence, there is evidence for both positive and negative correlations between cortisol and IgG levels in newborn animals.
The conflicting results, regarding the effects of dystocia and plasma cortisol on macromolecule absorption, may be explained by the fact that the maturational effects of cortisol on the intestinal epithelium are highly developmentally dependent (see earlier). In addition, there may be effects that are counteracting each other. Positive effects of "birth stress" on IgG absorption may be present mainly in premature animals having an immature epithelium at birth, while in term animals, the effects of birth stress are more likely to inhibit immunoglobulin uptake. Such effects of excessive birth stress could be mediated directly or indirectly via the dystocia-induced abnormal blood gas values (hypoxia, hypercapnia, acidosis), metabolite concentrations (low glucose, high lactate) or endocrine status (elevated cortisol, adrenalin). At present however, the available literature provides no conclusive evidence for these hypotheses and more research is needed to elucidate the effects of birth stress on the ability of the small intestine to absorb immunoglobulins. It remains however, that the reduction in IgG uptake, documented in some studies on dystocial newborn animals, is quantitatively small. This suggests that the process of birth per se does not exert a major influence on the timing and the extent of endocytosis in the newborn small intestine.
Growth retarded newborn animals
Intra-uterine growth retardation is a common problem observed among farm animals, particularly in species with large litters (e.g. pigs). The different factors that contribute to a low birth weight at term include genetic predisposition, poor maternal nutrition and fetal/maternal infection. Such influences may cause a sub-optimal supply of nutrients and oxygen to the developing fetus across the placental vascular beds. Due to the multi-factional etiology of the low birth weight syndrome, its effects on intestinal immunoglobulin absorption vary depending on the exact reason for fetal growth retardation.
It is not clear why growth-retarded newborn animals have an enhanced capacity to absorb macromolecules. Nevertheless, there are some general characteristics of fetal growth retardation that may exert a direct effect on the maturation of the small intestine, and its ability to absorb immunoglobulins. In particular, it appears that many fetuses born at term, but with low birth weight, have experienced prolonged hypoxic or nutritional stress in utero leading to a premature increase in adrenal secretion of cortisol which in turn induces premature maturation in all gut functions that are stimulated by cortisol . Hence, elevated cortisol levels in response to intra-uterine stress may explain the enhanced macromolecule uptake capacity in low birth weight animals. Stillborn pigs, and low birth weight pigs born alive, show histological characteristics in their adrenal glands indicative of prenatal exposure to stress and elevated cortisol levels [78, 8]. Correspondingly, elevated levels of corticotropin releasing hormone (CRH) and adrenocorticotropin (ACTH) have been detected in human fetuses born after intra-uterine growth retardation .
Although cortisol undoubtedly plays a role for the maturation of the small intestine, including its ability to absorb large molecules, the effects are highly age- and diet-dependent. In a recent study on fetal pigs , prevention of fetal swallowing by experimental gut obstruction was associated with both fetal body growth retardation and significantly elevated cortisol levels. Nevertheless, the ability of the small intestine to absorb large molecules by endocytosis, as measured in vitro, was not affected. Thus, the functional links among fetal growth retardation, plasma cortisol, and intestinal macromolecule absorptive capacity, remain obscure.
In vitro embryo production
Decreased viability and increased perinatal mortality is one of the drawbacks reported from offspring produced after transfer of in vitro produced (IVP) embryos in cattle reproduction. Larger calves at birth and a higher incidence of dystocia have been reported [48, 28, 85]. Factors influencing the viability of the newborn calf include maturation prior to birth, the stress of parturition and passive immunisation of the calf from colostrum . All of these factors have also been speculated to contribute to the lower viability of calves derived from IVP embryos but as yet, there are no clear evidence for physiological changes that can be directly linked to impaired survival. The results of IVP embryo production are highly variable among different laboratories, and differences in techniques used to perform in vitro embryo production complicate comparisons among studies. It remains, however, that calves produced after embryo cloning suffer more from abnormalities than calves produced after more conventional in vitro fertilization techniques, using only limited manipulation of the developing oocyte or embryo .
In studies on different groups of IVP calves,  found a lowered plasma IgG level in calves produced after a certain IVP method (co-culture of embryos without serum in the culture medium), but several lines of evidence suggest that the effect of IVP was small. Firstly, the serum IgG concentration was clearly above those considered to be necessary for passive immunisation and secondly, there was no difference between different IVP groups in the absorption efficiency of a non-IgG macromolecule, porcine serum albumin (PSA). Thirdly, the differences in mean IgG and PSA levels in plasma among different embryo treatments were quantitatively no greater than the differences associated with the variable degrees of birth dystocia as indicated by blood pH at birth. In fact, a major part of the variation in circulating IgG levels in newborn calves could be explained by the variable degree of dystocia rather than by the method of embryo production.
In the large farm animal species (horse, cattle, sheep, pig, goat), the newborn animals are normally born without systemic immunity in the form of immunoglobulins and passive transfer of maternal immunoglobulins from the first milk (colostrum) is required until the immune system has been fully developed. The degree of humoral immunological protection depends on the amount and the time of colostrum uptake, the colostral immunoglobulin quality and concentration, and the intestinal capacity to absorb immunoglobulins. Relatively little is known about the food and animal factors that influence the intestinal endocytotic capacity during the first 1–2 days after birth and the present review have described a series of factors that modulate this capacity in newborn animals. Firstly, the food in which the immunoglobulins are dissolved has a pronounced influence on the intestinal endocytotic capacity, and uptake is more efficient with colostrum from the same species than with a colostrum substitute. Secondly, an adequate maturation of the intestinal epithelium is crucial for an efficient intestinal transfer of large molecules. The functional maturation of the intestinal epithelium occurs very rapidly during the last weeks before term and therefore premature birth is associated with impaired immunoglobulin absorption. Other factors, including a lowered circulating level of cortisol, contributes to low immunoglobulin absorption in premature animals, particularly if the route of delivery is cesarean section. When birth occurs at full term the effects of mode of birth, and birth difficulties, on intestinal immunoglobulin absorption are less consistent. Correspondingly, the possible functional links among intestinal endocytotic capacity, plasma cortisol and blood chemistry (e.g. blood pH, oxygen, lactate, glucose) remain to a large extent unresolved. Finally, the literature indicates that the lowered neonatal survival associated with intra-uterine growth retardation (low birth weight) and in vitro production of embryos is not linked to a lowered capacity of the intestinal epithelium to absorb immunoglobulins. Hence, the nature of the feed (colostrum, milk, milk-replacer) and the stage of intestinal maturation appear to be the two most crucial factors affecting the epithelial capacity to absorb immunoglobulins in newborn farm animals. This information may help to improve the clinical care of 'compromised' newborns.
Many investigators involved in parts of the work carried out by the author are gratefully acknowledged. These include Mette Schmidt, Helene Jacobsen, Torben Greve, Jan Elnif, Jeff Trahair, Abigail Fowden, Marian Silver and Björn Weström. The author's own work has been sponsored by the Danish Agricultural and Veterinary Research Council and the NOVO Foundation.
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