The first report of Aelurostrongylus falciformis in Norwegian badgers (Meles meles)
© Davidson et al; licensee BioMed Central Ltd. 2006
Received: 02 May 2006
Accepted: 13 June 2006
Published: 13 June 2006
The first report of Aelurostrongylus falciformis (Schlegel 1933) in Fennoscandian badgers is described. Routine parasitological examination of nine Norwegian badgers, at the National Veterinary Institute during 2004 and 2005, identified A. falciformis in the terminal airways of five of the animals. The first stage larvae (L1) closely resembled, in size and morphology, those of Angiostrongylus vasorum (Baillet 1866). The diagnosis for both A. falciformis and A. vasorum is frequently based on the identification of L1 in faeces or sputum. The potential for misclassification of an A. falciformis infection as A. vasorum, where larval identification is the only diagnostic method used, is discussed.
Aelurostrongylus falciformis (Schlegel 1933) is a metastrongyle lung nematode of European badgers (Meles meles) and has been reported in continental Europe [1, 2] and Great Britain  but not Fennoscandia. Other lung nematodes seen in European badgers include the metastrongyles Angiostrongylus vasorum (Baillet 1866) [4, 5], Crenosoma sp. (Molin 1861) [1–4], Aelurostrongylus pridhami (Anderson 1962) , as well as the trichuroid nematode Capillaria aerophila (Creplin 1839) . A. vasorum is considered to be absent from the Scandinavian Peninsula. However, recently it has been found on the island of Sydkoster off the south west coast of Sweden  close to the Norwegian border. This parasite has its predilection site in the pulmonary artery and right ventricle of the heart. The diagnosis however, as for A. falciformis, is frequently based on the identification of first stage larvae (L1) in faeces or sputum . Crenosoma sp. and Capillaria aerophila infections can be differentiated from those of A. vasorum and A. falciformis on the basis of their typical L1 (Crenosoma sp.) and eggs (Capillaria aerophila).
Information regarding the nine badgers (Meles meles) submitted to the National Veterinary Institute, Oslo during the course of 2004 and 2005 for post-mortem examination and examined for lung worms.
Cause of death
Municipality of origin
The trachea and bronchi were dissected; two scrapes from the mucous membrane were taken and examined for nematode eggs and larvae. Biopsies, from each lung lobe, were fixed in 10% formalin and embedded in paraffin. They were then cut at 5μm and stained with haematoxylin and eosin for histological examination. The right side of the heart was incised and this incision extended along the pulmonary artery to look for adult A. vasorum. At least two 10 gram faecal samples per animal, from some of the badgers, were set up in Baermann apparatus for 6 hours and then examined for faecal larval burden, larvae per gram (lpg); the larvae collected were stored in 70% ethanol. Larvae from the respiratory tract and faeces were examined under magnification, those with a wavy tail were digitally photographed: length and width measurements were taken (Leica Image Manager IM50 Measurement Module, Version 4.0, Release 106). The width was measured at the widest point.
Nematode larvae with a curvy tail and a notch, suggestive of A. vasorum infection, were found for the first time in bronchial scrapes. A more advanced examination of the lungs in badger 1 was carried out. The lungs were dissected to bronchiolar level and any adult nematodes were carefully removed and placed in 70% ethanol. Larvae were extracted from the uterus of an adult female and compared to those found in the respiratory tract and faeces. The adult nematodes and larvae were examined under magnification, digitally photographed and measured. All adult nematodes fragmented during extraction, and their overall length could not be measured. Width measurements were taken just anterior to the spicules in the males and level with the uterus in the females. The length of the male spicules and accessory spicules were recorded.
Several dimensions of adults and larvae of Aelurostrongylus falciformis as found in Norwegian badgers (with the median value given in brackets), as well as analogous dimensions of A. falciformis and Angiostrongylus vasorum according to reference literature.
Aelurostrongylus falciformis – Norwegian badgers
Male width (μm)
48 – 81.5 (69)
170 – 235
Female width (μm)
84.5 – 148.5 (105)
60 – 172
220 – 306
95 – 133 (100)
400 – 500
Accessory spicule length (μm)
39 – 52.5 (46)
L1 larval length (μm)
213 – 353 (248)
220 – 370
310 – 399
L1 larval width (μm)
9 – 19 (14)
15 – 17
13 – 17
The present study is believed to be the first report of A. falciformis infection in Fenno-Scandinavian badgers. The L1 length was within the published reference range for A. falciformis, which overlaps the published L1 range for A. vasorum. A. vasorum can be found in ectopic locations , but the male nematodes, from the reported badger's lungs, had short spicules and an accessory chitinous piece, excluding A. vasorum from the differential list. Nevertheless, the great similarity between the L1 stages of A. falciformis and A. vasorum, in size and general morphology, needs to be considered.
The prevalence of A. falciformis found in European badgers, as well as the diagnostic criteria underlying the diagnosis, varies considerably between studies and needs further investigation. Magi et al. (1999)  identified adult A. falciformis and L1 in the lungs of 10 of 19 (53%) Italian badgers. In a British study of 118 badgers for tuberculosis, A. falciformis was recorded incidentally in one animal . In Spain, Millán et al. (2004)  reported A. vasorum larvae in the faeces of 24 of 26 (92%) badgers; however, they were unable to find adult nematodes in the pulmonary artery or the right ventricle of the heart. Another Spanish badger study  reported the presence of both A. vasorum and A. pridhami, the latter is a parasite of wild mink in North America , without describing the identification methods used. It may be possible that an infection with A. falciformis, particularly in areas where A. vasorum is endemic, could be misinterpreted as A. vasorum, when larval identification is the only diagnostic method used.
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