Zoonotic organisms such as viruses, bacteria or parasites can possess the potential to cause severe diseases both in humans and animals. Free-ranging animals with sporadic or indirect contact to domestic livestock and humans may serve as reservoirs or sentinels for diseases. Transmission of these pathogens can occur directly from a reservoir to the susceptible animal or human being, for example through direct contact with free-ranging animals including cervids [1]. Indirect transmission occurs via vectors, i.e. mosquitos, by contamination of the environment through faecal shedding [2] or by consumption of venison [3]. The epidemiological situation in free-ranging, semi-domesticated animals like reindeer (Rangifer tarandus tarandus), however, is difficult to assess, in contrast to domestic animals. Conclusions derived from studies which focus on domestic animal species in extensive husbandry systems can only be used with caution. But it can be assumed that pathogens are transmitted easily through close animal contact and lead to high animal losses as it is known from intensive husbandry systems. This is of special importance as the crowding of reindeer for winter feeding is becoming more and more common, particularly in southern parts of Northern Finland.

With regard to the occurrence of possibly zoonotic pathogens in reindeer, data is rare. In view of the zoonotic enteropathogens examined in this study, Campylobacter species, Enterococcus species, Escherichia coli, Salmonella species and Yersinia species are among the most important bacteria which cause severe enteric diseases. In detail, various Campylobacter species (C. coli, C. jejuni subspecies jejuni, C. hyointestinalis, C. lari and C. upsaliensis) were isolated from humans with gastro-enteritis [4, 5]. Campylobacter hyointestinalis was detected in healthy Finnish reindeer [6]. In a recent study about Campylobacter species and other bacteria in wild cervids in Norway, no Campylobacter species were detected in wild reindeer [7]. Enterococcus species are known as pyogenic organisms and can cause hospital acquired infections, but are primarily natural inhabitants of the gastrointestinal tract of humans and animals. Enterococcus species in reindeer have not yet been described. The virulence and pathogenesis of the examined bacteria depends on different factors. In E. coli, for instance, the ability to cause severe disease in humans and animals is associated with the occurrence of several virulence factors such as shigatoxins. Therefore, the presence of shigatoxin genes can indicate the virulence of certain strains, also known as shigatoxin-producing E. coli (STEC). In 50 faecal samples from wild Norwegian reindeer, 42 were positive for E. coli, but no shigatoxin-producing strains were identified [7]. Like STEC, Campylobacter species, Salmonella species and Yersinia species are a problem in meat production with a high infection risk for humans who consume contaminated products. Salmonella species have been found to be associated with mortality in reindeer in Finland and Sweden [8]. Among 153 wild Norwegian reindeer, no positive samples for Salmonella species were found [7]. Out of the former eleven known species of Yersinia, Y. pestis as the cause of plague, Y. pseudotuberculosis and certain biotypes of Y. enterocolitica are of great significance for human health. In Europe, the Y. enterocolitica biotypes O:3, O:9 and O:5,27 in particular are associated with human gastro-enteritis [9]. A novel species, Y. aleksiciae, identified by using 16S rRNA gene sequence type analysis and lysine decarboxylase (LDC) and in this points different from members of Y. kristensenii, was described by Sprague & Neubauer [10].

No data is available on the occurrence of zoonotic protozoa in Northern European reindeer such as Cryptosporidium species as a causative agent for heavy diarrhoea in humans and animals.

In 2,224 (99.2%) out of the total number of 2,243 faecal samples, one or more of the examined bacteria species were isolated.

There was no evidence of the occurrence of Salmonella species nor Cryptosporidium species. In total, 108 (4.8%) strains of Yersinia species were isolated, consisting of Y. enterocolitica biogroup 1A (n = 29), Y. intermedia (n = 2), Y. kristensenii (n = 72), Y. mollaretii (n = 3) and Y. rhodei (n = 2).

No significant differences were found for Enterococcus species and E. coli with regard to the degree of intensity of reindeer herding, the season or the geographic origin, whereas the prevalence of Yersinia species differed significantly (p ≤ 0,001): prevalence for Yersinia species in free-ranging reindeer in summer and autumn were significantly higher than in fenced reindeer during winter, as shown in Figure 1. A direct comparison of fenced (n = 100) and free-living (n = 147) reindeer during different seasons was possible in Näkkälä, but with prevalences of 100,00% (fenced) and 65,99% (free-living) for Enterococcus species, 85,00% and 91,63% for E. coli and 1,00% and 0 % for Yersinia species no significant differences could be detected.

In reindeer, Enterococcus species and E. coli occurred at very high prevalence, showing the affiliation of these two species to the normal intestinal flora of healthy reindeer. With regard to E. coli, there are few reports of diseases caused by shigatoxin-producing bacteria in ruminants [14, 15]. However, these bacteria are of extreme importance in causing severe diseases in humans [16]. As the genes encoding stx1, eae and hly _EHEC were detected in very few of the isolated E. coli-strains, the human health risk due to E. coli excreted by reindeer can be considered very low at present. These results comply with two other studies detecting no E. coli O157:H7 in 1,387 faecal and 421 meat samples from reindeer [17] and no STEC in 50 faecal samples from reindeer [7]. It is known, however, that STEC virulence factors are mobile within bacterial populations [18]. Therefore, an increase in the occurrence of toxin genes in E. coli from reindeer cannot be excluded when influencing parameters such as herding conditions are changed.

Yersinia species were isolated in 108 samples. The identified species Y. intermedia,Y. kristensenii, Y. mollaretii and Y. rhodei had been isolated before from various environmental samples, food, healthy animals and healthy and diseased humans [19, 20]. Even though these species are widely distributed in nature, their actual impact on human health is a matter of controversy. However, as they are isolated from persons with gastrointestinal disorders, the role of these species should not be disregarded [20]. These species seem to be well adapted to warm-blooded animals, including humans. After the identification of the novel species Y. aleksiciae [10], the isolates originally phenotyped as members of Y. kristensenii had to be investigated again (unpublished data). The isolated Y. enterocolitica strains belonged to biogroup 1A, which embraces the non-pathogenic European Y. enterocolitica strains, often isolated from environmental samples, foods, animal and human faeces [21]. Yersinia species, further analysed as Yersinia pseudotuberculosis serotype 1a, had also been isolated from reindeer before [22], but in samples from 35 Norwegian reindeer no Yersinia species were found [23]. The seasonal pattern of Yersinia species with an increase in the summer period can be attributed to the constant accidental incorporation of environmental strains which in this extent is not given in winter with limited survival conditions for bacteria.

Campylobacter hyointestinalis was isolated from one sample only. As the cultivation of Campylobacter species is difficult, the actual prevalence might be higher. Campylobacter hyointestinalis has hitherto been associated only sporadically with human gastrointestinal disorders [5, 24]. Even though the prevalence of Campylobacter species in this study was very low, it shows that reindeer can be carriers. This is supported by a study from Hänninen et al. [6] who detected Campylobacter hyointestinalis at a prevalence of 6% in 399 healthy Finnish reindeer. Lillehaug et al. [7] isolated no Campylobacter species in 150 faecal samples of wild reindeer.

Neither Salmonella species nor Cryptosporidium oocysts were detected in reindeer in this study. Both pathogens had been isolated from the environment, farm animals and humans in Fennoscandia [25, 26]. The occurrence of Salmonella species in Finnish reindeer is described by Kuronen et al. [8]. Regarding Salmonella species, the results of this study and of the study from Lillehaug et al. [7] with negative results for Salmonella species in 153 faecal samples from wild reindeer, indicate that wild cervids do not contract Salmonella-infections to any significant extent from other wildlife.

In Rangifer tarandus, a new genotype of Cryptosporidium closely related to Cr. serpentis, Cr. muris and Cr. andersoni, was isolated from 3 out of 49 caribou examined in Canada [27]. Concerning Northern European reindeer, no bibliographical references exist, but deriving from other species, the prevalence was expected to be higher in younger animals. This could not be supported by this study, identifying no Cryptosporidium oocysts at all.

All bacteria analysed in this study may be found in Northern Europe in the environment in aquatic, terrestrial and animal reservoirs [28] and have been isolated before from the intestinal tract of healthy or diseased ruminants world-wide [29, 30]. Even though most of the isolated bacteria strains do not have the potential to cause severe human or animal health problems, certain strains might be a risk, especially for immuno-supressed, old or very young persons and animals. Therefore, one has to regard the epidemiological impact of transmission of these infectious agents from the environment to reindeer and man and vice versa, depending on a number of local factors. To recapitulate, the excretion risk of pathogens by reindeer has to be considered in the context of the extreme climatic conditions in the research area. Permafrost soils of the tundra and taiga are a domain of psychrophilic and psychrotolerant organisms [31], but as enteropathogens, living in intestines of warm-blooded animals at 37°C, are not adapted to these extreme northern environmental conditions, fast destruction is probable.

In conclusion, the enteropathogens examined were either not detected at all (Salmonella species and Cryptosporidium species), in very small numbers (Campylobacter species) or if detected, their virulence and pathogenicity was very low (E. coli and Yersinia species). The potential human and animal health risk from reindeer excreting various important enteropathogenic bacteria and Cryptosporidium species should be regarded very low at present. No differences could be found in the flora between fenced and free-living animals. However, especially if reindeer are crowded, e.g. for winter feeding, an increased prevalence of enteric pathogens excreted by reindeer and eventually an increased risk to the consumer has to be considered as is already known from other intensive animals husbandry systems worldwide.

With respect to the investigated pathogens, the analysis of faecal samples from Norwegian and Finnish reindeer indicates that the animals do not represent an important source for zoonotic diseases at the moment. Enterococcus species and E. coli belong to the normal intestinal flora of reindeer. Climate conditions in the northern regions are a limiting factor to the survival of enteropathogens in the environment and might be a reason for the low prevalences of the other pathogens examined.

Campylobacter spp., Enterococcus spp., Escherichia coli, Salmonella spp., Yersinia spp., og Cryptosporidium oocyster hos semi-domesticerade renar (Rangifer tarandus tarandus) i Finlands och Norges nordliga trakter

Målsättningen med studien var värdering av zoonotiska enteropatogeners fekala utbredning hos semi-domesticerade renar (Rangifer tarandus tarandus) för att bedöma potentialla risker för männsikans hälsa med modern renskötsel. Sammanlagt 2243 träckprover från renar i Finlands och Norges nordliga trakter har analyserats på potentiellt enteropatogena bakterier (Campylobacter species, Enterococcus species, Escherichia coli, Salmonella species och Yersinia species) och parasiter (Cryptosporidium species) med standardiserade metoder. Escherichia coli har isolerats i 94.7%, Enterococcus species i 92.9%, Yersinia species i 4.8% av proverna och Campylobacter species i bara ett prov (0.04%). Analys av virolensfaktorer i E. coli och Yersinia species visade inga patogena egenskaper. Varken Salmonella species eller Cryptosporidium-oocystor har påvisats. Den allmänna hälsorisken gällande zoonotiska sjukdomar på grund av renskötseln bedöms för tillfället som mycket låg men risken kan öka om renskötselns villkor ändras med hänsyn till intensitet och hållande i hägn.