A field-study on Leptospira seroprevalence in dairy cows in four geographical areas in Sweden
© Lindahl et al; licensee BioMed Central Ltd. 2011
Received: 30 January 2011
Accepted: 12 October 2011
Published: 12 October 2011
Dairy cattle were used as sentinels for the presence of Leptospira infection in Swedish livestock in four regions contrasting in precipitation and temperature during the summer time. The aim of the study was to estimate the prevalence of five serovars of Leptospira of low pathogenicity in dairy cattle in these four regions.
Around 150 blood samples were collected from five dairy farms from each region, making 610 samples in total, during December 2009. The samples were screened for L. kirschneri sv Grippotyphosa, L. interrogans sv Icterohaemorrhagiae, L. interrogans sv Canicola, L. borgpetersenii sv Sejroe and one domestic strain similar to sv Sejroe, called strain Mouse 2A using the Microscopic Agglutination Test. Six animals (1%) were seropositive for the strain Mouse 2A. Four of the positive samples were from the south-west region which also was the region with highest precipitation. There were no positive samples to any of the other serovars studied.
The present data indicate that there is a low seroprevalence of Leptospira in Swedish dairy cows. These findings can be used as baseline data to investigate the effects of, for instance, climatic change or alterations in wildlife reservoir populations on the seroprevalence of Leptospira in the future.
Keywordscow Leptospira Sweden serology
The zoonosis leptospirosis, which is of worldwide distribution, is caused by different pathogenic serovars belonging to the genus Leptospira and is endemic in most tropical and temperate climates . The bacteria thrive in humid and warm climate and outbreaks of leptospirosis increase during heavy rainfall and flooding . Leptospirosis is therefore of particular interest for Europe in the context of climatic change and has been recommended for specific surveillance and control measures by the World Organization for Animal Health (OIE) . Similarly, the European Centre for Disease Control (ECDC) is also pinpointing leptospirosis as an important infectious disease of particular interest in Europe .
The severe pathogenic serovars Pomona in pigs and Hardjo in cattle have not been reported in Sweden . However, in pigs reared outdoors, seroprevalences of 4 and 1% for sv Bratislava and sv Icterohaemorrhagiae have been found (Boqvist S, Eliasson-Selling L, Bergström K, Magnusson U: Association between rainfall and temperature, and seroprevalence of Leptospira in outdoor reared pigs, submitted). The latter study also showed that the seroprevalence for a domestic strain called Mouse 2A (similar to sv Sejroe) was 8%. In Swedish cattle, the seroprevalence of serovars with lower virulence than sv Hardjo is virtually unknown.
In the present study, dairy cattle in farms with summer grazing were used as sentinels for possible differences in seropositivity between regions with contrasting climate but similar breeds and farming practices. The aim of the study was to estimate the prevalence of five serovars of Leptospira of low pathogenicity in dairy cattle in four climate regions in Sweden.
Materials and methods
Dairy herds of 40-60 cows were included to represent the average dairy herd size in Sweden . A list of ten eligible herds with between 25 and 80 dairy cattle from each region was provided by the Swedish dairy association, Stockholm. From this list five herds per region were selected, making 20 herds in total, based on i. their geographical location, since the aim was to distribute the included herds evenly within each region; ii. the willingness of the farmers to participate in the study; and iii. the housing system, since a pre-requisite for inclusion was that the cows should be reared in a tie-stall system to facilitate the sampling.
In total, 610 serum samples were collected, ranging from 25-33 samples per herd and 150-156 per region. The median number of dairy cows per herd was 50 (ranging from 25-80) and the median number of all cattle per herd was 104 (45-180). This was sufficient to estimate a within-herd seroprevalence of around 5%, depending on herd size, with an error of 5% and a confidence interval of 95% (Win Episcope 2.0). Fisher's exact test was used to compare seroprevalences between regions. Within the herds cows were selected randomly and sampled by the same person during December 2009. Blood samples were taken from the coccygeal vein and transported in a cooler to local laboratories for centrifugation. The samples were subsequently transported frozen to the Swedish University of Agricultural Sciences in Uppsala and kept at -20°C until analysed. The study was approved by decision C 322/9 at the Ethical Committees on Animal Experiments, Uppsala, Sweden. All sera were examined by the Microscopic Agglutination Test as described previously  for L. kirschneri sv Grippotyphosa, strain Duyster, L. interrogans sv Icterohaemorrhagiae, strain Kantorowicz, L. interrogans sv Canicola, strain Hond Utrecht IV, L. borgpetersenii sv Sejroe, strain M84 and L. borgpetersenii, domestic strain Mouse 2A isolated from the kidney of a mouse caught in a Swedish pig herd (Eva Olsson Engvall, National Veterinary Institute, Uppsala, Sweden). The other serovars were selected as they are known to be of clinical importance in cattle. Notably, the well known pathogenic sv Hardjo was not included as this serovar has been surveilled regularly for in Sweden without any positive findings . The cut-off value was 1:100. Of all sampled cows 65% were of the Swedish black and white breed (SLB) and 35% of the Swedish red and brown breed (SRB). The median age of all cows was 4 years (2-12). The median grazing period per year was 5 months (3.5- 6) and the dominant form of pasture was arable land (77%).
Results and Discussion
Six (1%) of the animals were serologically positive for the strain Mouse 2A. The titers were 1:100 in five animals and 1:200 in one. Four of these animals were from three farms in the south-west region, one animal in the mid-west region and the last one in the mid-east region. There were no statistically significant differences in seroprevalence between the regions. Both breeds were represented, each with three seropositive animals. All seropositive animals were in the age interval 3-6 years.
Most studies on Leptospira seroprevalence in livestock are from slaughter houses without any farm data. Here farm and metrological data from 20 herds and approximately 600 cows were included. This number of animals gives an indication of the serological situation in the southern part of Sweden, even though it may be difficult to measure low titers resulting from chronic infections by the MAT . Despite this difficulty, a previous study in pigs kept outdoors showed a seroprevalence of 8% against the strain Mouse 2A (Boqvist S, Eliasson-Selling L, Bergström K, Magnusson U: Association between rainfall and temperature, and seroprevalence of Leptospira in outdoor reared pigs, submitted). In the latter study the highest seroprevalence of Leptospira was found in farms in the rainier south- western region of Sweden and there was a positive association between seropositivity and rainfall. In the present study no differences in Leptospira seroprevalence in cattle between the regions could be demonstrated; however, it was not possible to take into account clustering of disease because of the design of the study. The present data suggest that seropositivity to pathogenic Leptospira in cows is low in Sweden and currently there are no reasons to believe that there are differences between regions attributable to factors such as temperature and precipitation. These findings can be used as baseline data to investigate potential future changes of Leptospira seroprevalence, such as climatic changes or alterations in wildlife reservoir populations.
All the farmers participating in this study are acknowledged for their willingness to participate. We also acknowledge the SMHI for their contribution of data on precipitation and temperature from the selected areas. The Swedish University for Agricultural Sciences' program for Environmental monitoring contributed financially to this study.
- Adler B, Moctezuma AP: Leptospira and leptospirosis. Vet Microbiology. 2010, 140: 287-296. 10.1016/j.vetmic.2009.03.012.View ArticleGoogle Scholar
- Levett PN: Leptospirosis. Clin Microbiol Rev. 2001, 14 (2): 296-326. 10.1128/CMR.14.2.296-326.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Ahern M, Kovats RS, Wilkinson P, Few R, Matthies F: Global Health Impacts of Floods: Epidemiologic Evidence. Epidemiol Rev. 2005, 27: 36-46. 10.1093/epirev/mxi004.View ArticlePubMedGoogle Scholar
- Dufour B, Moutou F, Hattenberger AM, Rodhain F: Global change: impact, management, risk approach and health measures - the case of Europe. Rev Sci Tech (International Office of Epizootics). 2008, 27: 529-550.Google Scholar
- Dujardin JC, Campino L, Cañavate C, Dedet JP, Gradoni L, Soteriadou K, Mazeris A, Ozbel Y, Boelaert M: Spread of vector-borne diseases and neglect of Leishmaniasis, Europe. Emerging Infect Dis. 2008, 14: 1013-1018. 10.3201/eid1407.071589.PubMed CentralView ArticlePubMedGoogle Scholar
- National Veterinary Institute: Surveillance and control programs. Domestic and wild animals in Sweden 2007. Edited by: Elvander M, Carlsson U. 2008, Uppsala, National Veterinary Institute Sweden, SVA report 4 ISSN 1654-7098Google Scholar
- Swedish Meteorological and Hydrological institute. [http://www.smhi.se]
- Swedish dairy association. [http://www.svenskmjolk.se]
- Båverud V, Gunnarsson A, Engvall EO, Franzén P, Egenvall A: Leptospira seroprevalence and associations between seropositivity, clinical disease and host factors in horses. Acta Vet Scand. 2009, 51: 15-10.1186/1751-0147-51-15.PubMed CentralView ArticlePubMedGoogle Scholar
- National Veterinary Institute: Regeringsuppdrag. Sjukdomsrapportering. 2006, Uppsala, National Veterinary Institute, Sweden, (in Swedish), [http://www.sva.se/upload/Redesign2011/Pdf/Om%20SVA/publikationer/Trycksaker/1/sv_reg_rapport2006_webb.pdf]Google Scholar
- Radostitis OM, Blood DC, Gay CC: Diseases caused by Leptospira spp. Veterinary Medicine - A text book of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 1994, London: Baillière Tindall, 884-907. 8Google Scholar
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