Implementation of immunohistochemistry on frozen ear notch tissue samples in diagnosis of bovine viral diarrhea virus in persistently infected cattle
© Bedekovićć et al; licensee BioMed Central Ltd. 2011
Received: 17 August 2011
Accepted: 5 December 2011
Published: 5 December 2011
Bovine viral diarrhea is a contagious disease of domestic and wild ruminants and one of the most economically important diseases in cattle. Bovine viral diarrhea virus belongs to the genus Pestivirus, within the family Flaviviridae. The identification and elimination of the persistently infected animals from herds is the initial step in the control and eradication programs. It is therefore necessary to have reliable methods for diagnosis of bovine viral diarrhea virus. One of those methods is immunohistochemistry. Immunohistochemistry on formalin fixed, paraffin embedded tissue is a routine technique in diagnosis of persistently infected cattle from ear notch tissue samples. However, such technique is inappropriate due to complicated tissue fixation process and it requires more days for preparation. On the contrary, immunohistochemistry on frozen tissue was usually applied on organs from dead animals. In this paper, for the first time, the imunohistochemistry on frozen ear notch tissue samples was described.
Seventeen ear notch tissue samples were obtained during the period 2008-2009 from persistently infected cattle. Samples were fixed in liquid nitrogen and stored on -20°C until testing. Ear notch tissue samples from all persistently infected cattle showed positive results with good section quality and possibility to determinate type of infected cells.
Although the number of samples was limited, this study indicated that immunohistochemistry on formalin fixed paraffin embedded tissue can be successfully replaced with immunohistochemistry on frozen ear notch tissue samples in diagnosis of persistently infected cattle.
Bovine viral diarrhea is contagious disease of domestic and wild ruminants and one of the economically most important diseases in cattle. Bovine viral diarrhea virus (BVDV) belongs to the genus Pestivirus, within the family Flaviviridae . Based on viral RNA sequence, there are at least 2 viral genotypes of BVDV that can be further divided into subgenotypes [2, 3].
Acknowledgements and funding
This research was supported by grant No. 048-0481186-1183 from the Ministry of Science, Education and Sports, Republic of Croatia.
- Heinz FX, Collett MS, Purcell RH, Gould EA, Howard CR, Houghton M, Moormann RJM, Rice CM, Thiel HJ: Genus pestivirus. Virus Taxonomy. Edited by: Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carsrens E, Estes MK, Lemon S, Maniloff J, Mayo MA, Mcgeogh D, Pringle CR, Wickner RB. 2000, New York: Academic press, 867-872.Google Scholar
- Flores EF, Ridpath JF, Weiblen R, Vogel FS, Gil LH: Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: Evidence for a subgenotype within BVDV-2. Virus Res. 2002, 87: 51-60. 10.1016/S0168-1702(02)00080-1.View ArticlePubMedGoogle Scholar
- Vilček S, Durkovič B, Kolesarova M, Greiser-Wilke I, Paton D: Genetic diversity of international bovine viral diarrhoea virus (BVDV) isolates: identification of a new BVDV-1 genetic group. Vet Res. 2004, 35: 609-615. 10.1051/vetres:2004036.View ArticlePubMedGoogle Scholar
- Lee KM, Gillespie JH: Propagation of virus diarrhea virus of cattle in tissue culture. Am J Vet Res. 1957, 18: 952-953.PubMedGoogle Scholar
- Underdahl NR, Grace OD, Hoerlein AB: Cultivation in tissue-culture of cytopathogenic agent from bovine mucosal disease. Proc Soc Exp Biol Med. 1957, 94: 795-797.View ArticlePubMedGoogle Scholar
- Roeder PL, Jeffrey M, Cranwell MP: Pestivirus fetopathogenicity in cattle: Changing sequelae with fetal maturation. Vet Rec. 1986, 118: 44-48. 10.1136/vr.118.2.44.View ArticlePubMedGoogle Scholar
- Houe H: Epidemiological features and economical importance of bovine viral diarrhoea virus (BVDV) infections. Vet Microbiol. 1999, 64: 135-144. 10.1016/S0378-1135(98)00265-X.View ArticleGoogle Scholar
- Goyal SM: Diagnosis. Bovine Viral Diarrhea Virus. Diagnosis, Management, and Control. Edited by: Goyal SM, Ridpath JF. 2005, Ames, Iowa. Blackwell Publishing, 197-208. 1View ArticleGoogle Scholar
- Ridpath JF, Hietala SK, Sorden S, Neill JD: Evaluation of the reverse transcription-polymerase chain reaction/probe test of serum samples and immunohistochemistry of skin sections for detection of acute bovine viral diarrhea infections. J Vet Diagn Invest. 2002, 14: 303-307. 10.1177/104063870201400405.View ArticlePubMedGoogle Scholar
- Toplak I, Sandvik T, Barlič Maganja D, Grom J, Paton DJ: Genetic typing of bovine viral diarrhoea virus: most Slovenian isolates are of genotypes 1d and 1f. Vet Microbiol. 2004, 99: 175-185. 10.1016/j.vetmic.2003.12.004.View ArticlePubMedGoogle Scholar
- Cornish TE, Van Olphen AL, Cavander JL, Edwards JM, Jaeger PT, Vieyra LL, Woodard LF, Miller DR, Otoole D: Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. J Vet Diagn Invest. 2005, 17: 110-117. 10.1177/104063870501700203.View ArticlePubMedGoogle Scholar
- Hilbe M, Stalder H, Peterhans E, Nussbaumer M, Egli C, Schelp C, Zlinszky K, Ehrensperger F: Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. J Vet Diagn Invest. 2007, 19: 28-34. 10.1177/104063870701900105.View ArticlePubMedGoogle Scholar
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