Table 1 Antibodies used for immunohistochemistry on paraffin and/or frozen sections

mAbpool

1:2000^a

Mouse IgG1 and IgM

M. bovis

0.25% trypsin (37°C, 60 min)

Chemicon, Temecula, CA, USA

1A1

1:200^a/1:800^b

Mouse IgG1

M. bovis Vsp^a A, C

0.25% trypsin (37°C, 60 min)

[46]

1E5

u.d.^c/1:70^b

Mouse IgM

M. bovis Vsp^a A, B, C

0.25% trypsin (37°C, 60 min)

[47]

I₂

1:900^b

Mouse IgG1

M. bovis pMB67^b

w/o^a

[7, 48]

CC30

1:5^b

Mouse IgG1

Bovine CD4

w/o^a

Biozol, Eching, Germany

CC63

1:50^b

Mouse IgG2a

Bovine CD8

w/o^a

Biozol, Eching, Germany

A10-116F^a

1:8000^a

Sheep IgG

Bovine IgG1

0.05% pronase E (37°C, 20 min)

Bethyl Laboratories, Montgomery, TX, USA

A10-117^a

1:3000^a

Sheep IgG

Bovine IgG2

0.05% pronase E (37°C, 20 min)

Bethyl Laboratories, Montgomery, TX, USA

A10-100F^a

1:400^a

Rabbit IgG

Bovine IgM

0.05% pronase E (37°C, 20 min)

Bethyl Laboratories, Montgomery, TX, US

A10-108F^a

1:4000^a

Rabbit IgG

Bovine IgA

0.05% pronase E (37°C, 20 min)

Bethyl Laboratories, Montgomery, TX, USA

AE1/AE3

1:500^a

Mouse IgG

Wide spectrum-cytokeratin

0.05% pronase E (37°C, 20 min)

DakoCytomation, Hamburg, Germany

TAL.1B5

1:4000^a

Mouse IgG1

α-chain of human leukocyte antigen (HLA-DR)

0.01 M citric buffer (microwave 95°C, 15 min)

DakoCytomation, Hamburg, Germany