Table 3 Sequences of oligonucleotide probes printed onto the microarray
From: DNA microarray-based detection of Coxiella burnetii, the causative agent of Q fever
Probe | Target gene | Sequence (5’ → 3’) | Tm (°C) | GC (%) | Length (nt) |
|---|---|---|---|---|---|
1a | icd | CCTGACCGACCCATTATTCCCTTTATCGAAGGAG | 66,1 | 50,0 | 34 |
1b | CCTGACCGACCCATTATTCCCTTTATCGAAGG | 65,1 | 50,0 | 32 | |
1c | CCCATTATTCCCTTTATCGAAGGAGATGGGATTGG | 64,9 | 46,0 | 35 | |
2a | omp/com1 | CGTGGCGATAGCCGCCCCCTCTCAAT | 69,3 | 65,0 | 26 |
2b | CTCCTCAACAAGTCAAAGACATACAAAGCATCG | 63,3 | 42,0 | 33 | |
2c | CAAAGCATCGTCCACCATTATTTAGTCAACC | 62,0 | 42,0 | 31 | |
3a | IS1111 | GTAAAGTGATCTACACGAGACGGGTTAAGCG | 64,0 | 48,0 | 31 |
3b | GCTCAGTATGTATCCACCGTAGCCAGTCTTAAG | 64,6 | 48,0 | 33 | |
3c | CTGCGTGGTGATGGAAGCGTGTGGAG | 66,9 | 62,0 | 26 | |
4a | cbbE | CTAAGAAACTGCTTAAGAGAGGGCAGGGAACG | 65,3 | 50,0 | 32 |
4b | GGGCAGGGAACGATAGTGTGTTGCGGTATTTAC | 67,3 | 52,0 | 33 | |
4c | CTTGAAAAACGTAGCGGAAAAGGGACAGTACG | 65,0 | 47,0 | 32 | |
5a | cbhE | GCTTATTTCGCCCTCGCTGACGGAAGAGGATC | 68,6 | 56,0 | 32 |
5b | CGGAAGAGGATCTTTTAGGTCGAATTAGACAGAGATAC | 64,0 | 42,0 | 38 | |
5c | GCTGGGGGCCAAGAAAAGTTTGCAGACCAG | 68,5 | 57,0 | 30 | |
H | HybCtr | GACTTACCAACACATCAAAGTTCCCAGC | 61,7 | 46,0 | 28 |