Table 4 Primers used for amplification of target genes and hybridisation control
From: DNA microarray-based detection of Coxiella burnetii, the causative agent of Q fever
Name | Target gene | Sequence (5’ → 3’) | PCR product size (bp) | Tm (°C) | GC (%) | Length (nt) |
|---|---|---|---|---|---|---|
AT_ICD-F | icd | CGGAGTTAACCGGAGTATCCATC | 178 | 57,6 | 52,0 | 23 |
AT_ICD-R | GCATCGACCACGTTTTTCATG | 56,2 | 48,0 | 21 | ||
AT_Omp-F | omp/com1 | GCACTATTTTTAGCCGGAACC | 144 | 54,9 | 48,0 | 21 |
AT_Omp-R | TGCTTCTACTAAAACTTCTGGG | 53,0 | 41,0 | 22 | ||
AT_IS-F | IS1111 | CTGTGTGGAATTGATGAGTGG | 142 | 54,2 | 48,0 | 21 |
AT_IS-R | ACGTCCGATACCAATGGTTC | 55,0 | 50,0 | 20 | ||
AT_CbbE-F | cbbE | TAAGGGACATCCACTACCGG | 147 | 55,5 | 55,0 | 20 |
AT_CbbE-R | CCCAAATTTAGATCGTCACATTG | 53,6 | 39,0 | 23 | ||
AT_CbhE-F | cbhE | CGATGTCAACTCTAGAGAGC | 147 | 52,5 | 50,0 | 20 |
AT_CbhE-R | GCAATCTGCTCGGCAATAAAG | 55,6 | 48,0 | 21 | ||
HybCtr-F | ICD (HybCtr) | CGGAGTTAACCGGAGTATCCATC | 95 | 57,6 | 52,0 | 23 |
HybCtr-R | AACTTCTAAAACGGCTTTATTAAC | 51,5 | 29,0 | 24 |