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Luteinizing Hormone (LH) Response to Different Doses of Synthetic Gonadotropin Releasing Hormone (Gnrh) in Prepubertal Gilts

Plasmanivåerna av luteiniserande hormon efter olika doseringar av ett syntetiskt gonadotropin-releasing hormon hos prepubertala gyltor

Acta Veterinaria Scandinavica volume 27, pages 257–266 (1986)Cite this article

Abstract

The object of this investigation was to study luteinizing hormone (LH) response to different doses of synthetic gonadotropin-releasing hormone (GnRH) in prepubertal gilts. Four crossbred prepubertal gilts, 128–134 days old and body weight 57–63 kg, were used in this study. Four doses, 0. 5, 25 and 125 μg, of GnRH were administered via a jugular vein catheter in a latin square design. Each treatment consisted of 3 injections at 90 min intervals. Frequent blood samples were taken during a period of 90 min before up to 90 min after treatment. Total LH responses were measured from post-treatment samples as the area under the curve above base level obtained from pre-treatment samples. A positive relationship between GnRH dose and LH release was obtained in all gilts, except for 1 treatment given to a gilt with high plasma level of oestradiol-17β on the day of treatment. This study has demonstrated the responsiveness of the pituitary gland by LH release to different doses of GnRH in 4.5-month-old prepubertal gilts.

Sammanfattning

Målsättningen med denna undersökning var att studera frisättninigen av LH, efter injektion av olika doser av GnRH, hos prepubertala gyltor. Fyra korsningsgyltor, 128–134 dagar gamla och med en kroppsvikt av 57–63 kg, användes i försöket. Fyra olika doseringar av GnRH — 0, 5, 25 respektive 125 µg enligt “latin square design” — injicerades via en permanent jugular-venkateter. Varje behandling omfattade tre injektioner givna med 90 minuters intervall. Upprepade blodprover togs under tidsperioden 90 minuter före första injektionen till 90 minuter efter tredje injektionen. LH-svaret redovisas som ytan under LH-kurvan från en baslinje uträknad med ledning av LH-nivån före GnRH-behandlingen. Ett positivt samband påvisades mellan GnRH-dosen och LH-svaret hos samtliga djur och behandlingstillfällen med ett enda undantag. Vid detta tillfälle var östradiol-17β nivån i blodet hög. Resultaten av denna studie visar att hypofysens LH-frisättning är relaterad till mängden injicerat GnRH hos prepubertala gyltor.

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Acknowledgments

This work was supported by grants from the Swedish Council for Forestry and Agricultural Research. A scholarship awarded to S. Rojanasthien by the Swedish International Development Authority (SIDA) is also acknowledged.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, and Department of Clinical Chemistry, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden

    S. Rojanasthien, A. Kunavongkrit & S. Einarsson

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  1. S. Rojanasthien
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  2. A. Kunavongkrit
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  3. S. Einarsson
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Rojanasthien, S., Kunavongkrit, A. & Einarsson, S. Luteinizing Hormone (LH) Response to Different Doses of Synthetic Gonadotropin Releasing Hormone (Gnrh) in Prepubertal Gilts. Acta Vet Scand 27, 257–266 (1986). https://doi.org/10.1186/BF03548170

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Acta Veterinaria Scandinavica

ISSN: 1751-0147