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Salmonella serotypes in wild boars (Sus scrofa) hunted in northern Italy
Acta Veterinaria Scandinavica volume 55, Article number: 42 (2013)
Salmonella species (spp.) are zoonotic enteric bacteria able to infect humans, livestock and wildlife.
However, little is known about the prevalence and the presence of the different serovars in wildlife. Considering the wide distribution of wild boars and the feeding behaviour (omnivorous scavengers), wild boars may be a good indicator for environmental presence of Salmonella spp. The aims of this study were to determine the presence of Salmonella spp. in hunted wild boars and to determine the serotype the isolated strains.
Over three hunting seasons, the intestinal contents of 1,313 boars hunted in northern Italy were sampled and cultured. Salmonella spp. were isolated from 326 boars (24.82%). Thirty different serovars belonging to three different S. enterica spp. were found. Twenty-one serovars of S. enterica subsp. Enterica were found including the human pathogens S. Typhimurium and S. Enteritidis. In addition, nine serovars belonging to S.enterica subsp. diarizonae and S. enterica subsp. houtenae were detected.
Considering the widespread occurrence of wild boars in Europe, the epidemiological role of this species in relation to salmonellosis might be relevant and should be further investigated. Wild boars may act as healthy carriers of a wide range of Salmonella serotypes.
Salmonellosis was the second most common reported cause of zoonotic diseases in humans in Europe in 2010 where 99,020 cases were confirmed. Also, salmonellosis causes significant economic losses to the livestock industry [1, 2]. Indeed, Salmonella species (spp.) are able to infect a wide range of domestic and wild animal species and have been isolated from the intestinal content of birds and mammals [3–5] including wildlife such as white-tailed deer , rabbits and wild boars (Sus scrofa) .
The occurrence of wild boar populations in most of Europe, including Italy, has required increased hunting to limit their expansion. It has been suggested, that wild boars might represent an important reservoir for zoonotic pathogens, including Salmonella spp. , and thus may act as a source of food borne infections in humans. Moreover, wild boars are omnivorous scavengers and they forage on almost anything including carrion, insects, and reptiles. Bacterial pathogens ingested by this behaviour may colonize the intestinal tract and especially in areas with intensive animal farming, wild boars are likely to be exposed to pathogens of farm animal origin, such as S. Enteritidis and S. Typhimurium. In contrast to the abundance of literature on the prevalence of Salmonella spp. in humans and in domestic pigs, little is known about the occurrence of this pathogen in wild boars . In particular, limited data is available on the presence of the different serovars of Salmonella spp. Therefore, the aims of this study were to determine the prevalence of Salmonella spp. in wild boars hunted in northern Italy and to serotype the isolated strains.
Thanks to a health monitoring plan of free-ranging animals established in the Province of Brescia, Lombardy in northern Italy from 2007 to 2010, a total of 1,313 wild boars were sampled. In the three hunting season, 2007–2008, 2008–2009 and 2009–2010, the numbers of samples collected were 443, 442 and 428, respectively. During evisceration of killed boars, the large intestines were collected by hunters and delivered daily to the laboratory. For each animal the sex and age were registered. The age of the animals was determined on the basis of tooth eruption pattern : individuals were considered “young” when <12 months of age, “sub-adults” when 13–24 months of age and “adults” when >24 months of age. The statistical analysis was performed by Yates’ chi-square test for comparisons of sex and age classes, using R 2.11.0 software .
The isolation method  was based on the pre-enrichment phase where 25 g of intestinal contents were inoculated into sterile sampling bags with 225 ml of buffered peptone water and incubated at 37°C for 24 hours; thereafter, 0.1 ml was inoculated on Modified Semisolid Rappaport Vassiliadis (MSRV; Oxoid, Hampshire, UK). Following incubation for 48 h at 41.5°C, Salmonella suspected colonies on MSRV plates were plated on two selective solid media: Xylose Lysine Deoxycholate agar (XLD; bioMérieux, Bagno a Ripoli, IT) and Brilliant Green Agar (BGA; Vacutest Kima, Arzergrande, IT). All presumptive Salmonella spp. isolates that developed colonies on XLD or on BGA were confirmed using biochemical tests (BBL™ Enterotube™ II, Becton Dickinson, Heidelberg, DE).
Salmonella spp. were isolated from 326 wild boar intestines (24.82%). In the hunting seasons of 2007/08, 2008/09 and 2009/10, 124 (27.99%), 80 (18.05%) and 122 (28.50%) samples tested positive, respectively (Table 1). This average high prevalence of positive animals (24.83%) was unexpected considering that salmonellosis in the European population of wild boars is considered uncommon . A similar prevalence (22.1%) was found only recently in Portugal by analysing 77 wild boars, but just two serotypes (S. Typhimurium and S. Rissen) were identified . Salmonella spp. has also been isolated in Switzerland from the tonsils and faeces of wild boars with a prevalence of 5.5%, and three serovars were identified .
Out of 326 positive animals, 152 (46.62%) were males and 158 (48.46%), were females while the sex was not recorded for 16 (4.92 %) animals. Prevalence did not differ between males and females (χ2= 1.40, df=1, P = 0.23). However, young animals (yearlings) seem to be more susceptible to intestinal colonization by Salmonella spp. than older animals (χ2= 79.53, df = 2, P < 0.001) (Table 1).
Salmonella spp. isolations were never associated with presence of macroscopic lesions during necropsy. This is different from observations of farmed wild boars where some serotypes of Salmonella such as S. Choleraesuis and S. Saintpaul are known to cause multifocal to diffuse fibrinonecrotic typhlocolitis [12, 13].
Complete serological characterization of the 326 Salmonella strains was performed. This included rapid slide agglutination test for the detection of somatic antigens (Statens Serum Institut, Copenhagen, Denmark) and the hot tube agglutination test (Becton Dickinson, Heidelberg, Germany) according to Morris et al. for the determination of flagellar antigen. The results of the antigen determination were used for the final serological characterization using the White -Kauffmann - Le Minos scheme . The 326 Salmonella isolates belonged to 30 different serovars classified into three different subspecies of S. enterica: S. enterica subsp. enterica (n=259 strains, 79.45%, 21 serovars), S. enterica subsp. diarizonae (n=38, 11.66%, 5 serovars) and S. enterica subsp. houtenae (n=29, 8.90%, 4 serovars) (Table 2).
According to the nomenclature , the Salmonella enterica cluster is divided into six subspecies each containing a variable number of serovars. In this study, three subspecies were isolated. S. enterica subsp. enterica is considered the most widespread and includes many serovars, usually found in mammals, food and also in the environment . A total of 259 isolates of S. enterica subsp. enterica belonging to 21 different serovars (Table 2) were identified during the three hunting seasons. In particular, 11 serovars were detected in just one season, two serovars in two seasons and eight in all three seasons. Such high variability might indicate different sources of exposure from activities such as livestock farming and waste disposal, or exposure from other wild species such as birds and amphibians . S. Typhimurium was identified in all three seasons of this study. S. Typhimurium was the second most frequently identified S. enterica subsp. enterica serovar in European human salmonellosis cases reported in 2010 , and it deserves special attention due to its virulence and high prevalence of antibiotic resistance . Similar importance should also be given to both S. Enteritidis, which was the most frequently identified serovar in European human salmonellosis cases in 2010 and to S. Derby, which was included in the top 10 serovars reported in human cases in 2010 in Europe . The isolation of a relatively high number of S. enterica subsp. enterica serovars and their relative frequencies (S. Coeln 21.78%, S. Ball 7.67%, S. Thompson 7.06%, S. Veneziana 6.75% and S. Napoli 5.83%) prove how wild boars can consistently be indicators of the presence of a great variety of serotypes in the environment. It should be underlined that information in the literature on the identification, distribution and pathogenic role of S. Coeln is very scarce, though this serotype was considered the cause of an outbreak in humans in France . In addition, the isolation of S. Napoli and of S. Enterica 4,5,12:i:-, which is a monophasic variant of S. Typhimurium, might contribute to the understanding of the epidemiology and distribution of these serotypes, which are considered emerging serovars both in humans and animals [19, 20]. Further molecular investigation and the use of a more sensitive method such as pulsed-field gel electrophoresis (PFGE)  for fingerprinting Salmonella serovars, would be necessary to correctly classify the various strains, but it is beyond the aim of this study.
Other isolates from wild boars belonged to S. enterica subsp. diarizonae and S. enterica subsp. houtenae. According to the Enternet network report such serovars were not detected during official livestock surveys carried out in the same area over the same time period (data not published).
The risks factors for human salmonellosis are numerous: infection may indirectly arise in agricultural areas from the contamination of vegetable products, through direct animal contact, during hunting and carcass manipulation, or directly from ingestion of contaminated meat or meat products . The contamination of wild boar carcasses by intestinal contents containing enteric pathogenic bacteria could not be excluded in hunted animals.
The results are in agreement with the hypothesis that, although domestic animals are the major reservoir of Salmonella spp., the wild boars omnivorous feeding habits makes this animal more exposed to intestinal contamination and possible colonization by Salmonella spp. In addition, the potential role of wild boars as carriers and faecal spreaders of Salmonella spp. in the natural epidemiological cycle of this enteric pathogen must be highlighted. In this sense wild boars can act as a good sentinel species for the presence of environmental Salmonella serotypes, some of which have zoonotic implications.
Gortàzar C, Ferroglio E, Hofle U, Frolich K, Vincente J: Diseases shared between wildlife and livestock: a European perspective. Eur J Wildl Res. 2007, 53: 241-256. 10.1007/s10344-007-0098-y.
European Food Safety Authority (EFSA): The community summary report on trends and sources of zoonoses, zoonotic agents, and food-borne outbreaks in the European union in 2010. E.F.S.A. Journal. 2012, 10: 2597-
Refsum T, Handeland K, Baggesen DL: Salmonellae in avian wildlife in Norway from 1969 to 2000. Appl Environ Microb. 2002, 68: 5595-5599. 10.1128/AEM.68.11.5595-5599.2002.
Wahlström H, Tysen E, Olsson Engvall E, Brändström B, Eriksson E, Mörner T, Vågsholm I: Survey of campylobacter species, VTEC O157 and salmonella species in Swedish wildlife. Vet Rec. 2003, 153: 74-80. 10.1136/vr.153.3.74.
Millán J, Aduriz G, Moreno B, Juste RA, Barral M: Salmonella isolates from wild birds and mammals in the Basque country (Spain). Rev Sci Tech Off Int Epiz. 2004, 23: 905-911.
Renter DG, Gnad DP, Sargeant JM, Hygnstrom SE: Prevalence and serovars of salmonella in feces of free-ranging white-tailed deer (odocoileus virginianus) in Nebraska. J Wildl Dis. 2006, 42: 699-703.
Vieira-Pinto M, Morais L, Caleja C, Themudo P, Torres C, Igrejas G, Poeta P, Martins C: Salmonella sp. In game (Sus scrofa and oryctolagus cuniculus). Foodborne Pathog Dis. 2011, 8: 739-740. 10.1089/fpd.2010.0742.
Wacheck S, Fredriksson-Ahomaa M, König M, Stolle A, Stephan R: Wild boars as an important reservoir for foodborne pathogens. Foodborne Pathog Dis. 2010, 7: 307-312. 10.1089/fpd.2009.0367.
Saez-Royuela C, Gomariz C, Telleria JL: Age determination of European wild boar [Sus scrofa]. Wildl Soc Bull. 1989, 17: 326-329.
R Development Core Team: R: a language and environment for statistical computing. 2010, Vienna: R Foundation for Statistical Computing
International Organization for Standardization (ISO): Microbiology of food and animal feeding stuff—horizontal method for the detection of salmonella. ISO 6579. 2002, Geneva, Switzerland: International Organization for Standardization
Ecco R, Guedes RMC, Tury E, Santos HL, Perecmanis S: Outbreak of enterocolitic salmonellosis on a wild pig farm. Vet Rec. 2006, 158: 242-243. 10.1136/vr.158.7.242.
Perez J, Astorga R, Carrasco L, Mendez A, Perea A, Sierra MA: Outbreak of salmonellosis in farmed European wild boars [Sus scrofa ferus]. Vet Rec. 1999, 145: 464-465. 10.1136/vr.145.16.464.
Morris GK, Steele CD, Wells JG: Evaluation of plastic multi-well plates for serological screening of salmonella cultures with Spicer-Edwards pooled Antisera. Appl Microbiol. 1972, 24: 846-848.
Grimont PAD, Weill FX: Antigenic formulae of the salmonella serovars [. WHO collaborating center for reference and research on salmonella. 2007, Paris: Institut Pasteur,http://www.pasteur.fr/ip/portal/action/WebdriveActionEvent/oid/01s-000036-089, 9,
Paulsen P, Smulders FJM, Hilbert F: Salmonella in meat from hunted game: a central Europe perspective. Food Res Int. 2012, 45: 609-616. 10.1016/j.foodres.2011.06.055.
Cruchaga S, Echeita A, Aladueña A, García-Peña J, Frias N, Usera MA: Antimicrobial resistance in salmonellae from humans, food and animals in Spain in 1998. J Antimicrob Chemother. 2001, 47: 315-321. 10.1093/jac/47.3.315.
Haeghebaert S, Vaillant V, Portal H, Bouvet P, Minet JC, Grimont F: Epidemic of salmonellosis due to salmonella enterica serotype coeln. France, November 1998. Bulletin Épidémiologique Hebdomadaire. 2000, 36: 151-153.
Graziani C, Busani L, Dionisi AM, Caprioli A, Ivarsson S, Hedenström I, Luzzi I: Virulotyping of salmonella enterica serovar Napoli strains isolated in Italy from human and nonhuman sources. Foodborne Pathog Dis. 2011, 8: 997-1003. 10.1089/fpd.2010.0833.
Switt AI, Soyer Y, Warnick LD, Wiedmann M: Emergence, distribution, and molecular and phenotypic characteristics of salmonella enterica serotype 4,5,12:i:-. Foodborne Pathog Dis. 2009, 6: 407-415. 10.1089/fpd.2008.0213.
Murase T, Okitsu T, Suzuki R, Morozumi H, Matsushima A, Nakamura A, Yamai S: Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for salmonella infections. Microbiol Immunol. 1995, 39: 673-676.
The authors gratefully acknowledge the local hunters (Brescia Province, Northern Italy); without their cooperation these studies would not have been possible.
The authors wish to thank Dr. Lesley Benyon (ScienceDocs, Inc.) for the editing of this manuscript.
The authors declare no competing interests.
MC designed the study, performed the isolation of Salmonella spp. and drafted the first version of the manuscript. MZ initiated the study and supervised the isolation of Salmonella spp. and the statistical analysis. ST performed the serological characterization of the isolates. AL and LA supervised in the design of the study and the epidemiological analysis. All authors have read and accepted the final manuscript.
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Chiari, M., Zanoni, M., Tagliabue, S. et al. Salmonella serotypes in wild boars (Sus scrofa) hunted in northern Italy. Acta Vet Scand 55, 42 (2013). https://doi.org/10.1186/1751-0147-55-42
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