Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A
© Stenfeldt et al.; licensee BioMed Central Ltd. 2014
Received: 17 February 2014
Accepted: 16 June 2014
Published: 12 July 2014
Myocarditis is often cited as the cause of fatalities associated with foot-and-mouth disease virus (FMDV) infection. However, the pathogenesis of FMDV-associated myocarditis has not been described in detail. The current report describes substantial quantities of FMDV in association with a marked mononuclear inflammatory reaction, interstitial edema and cardiomyocyte degeneration in the myocardium of two pigs that died during acute infection with either of two different strains of FMDV. Despite similar clinical progression, there was a marked variation in morphological characteristics of myocarditis with a significant difference in intensity of myocardial inflammation between the two cases. Phenotypic characterization of leukocyte populations revealed that in both cases, the inflammatory infiltrate consisted mainly of combinations of CD172a+, CD163+ and CD44+ cells, with a distinct subset of CD8+ cells, but with consistent lack of detection of CD3+ and CD21+ cells. This suggests that the FMDV-associated acute myocardial inflammation in the two observed cases consisted mainly of leukocytes of monocyte lineage, with a distinct population of CD8+ cells which, based on lack of CD3 detection in serial sections, are likely to represent NK cells.
Foot-and-mouth disease (FMD) is a highly contagious vesicular infection of cloven-hoofed animals caused by FMD virus (FMDV), a single stranded positive sense RNA virus of the Picornavirus family. FMD is generally a disease that causes high morbidity and low mortality. However, there are sporadic reports of FMD outbreaks with unusually high mortality rates [,], with fatalities often being attributed FMDV-associated myocarditis (as reviewed by []). Investigations based on specimens obtained from field outbreaks [,], as well as experimental studies [,], have confirmed a relationship between acute FMDV infection and fatal myocarditis in young ruminants and pigs.
Although commonly reported as the cause of fatalities associated with this disease, detailed investigations into the pathogenesis of FMDV myocarditis are lacking. The current report presents virological and histopathological findings in the myocardium of two pigs that died during acute infection with FMDV strains A24 Cruzeiro (case 1) or O1 Campos (case 2). The association between the pigs’ deaths and the presence of FMDV in myocardial tissue samples was confirmed through virus isolation, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunomicroscopy. Myocarditic properties of the two FMDV strains were investigated through routine histopathological evaluation in combination with phenotypic characterization of inflammatory infiltrates.
This report is based on data obtained from two pigs (castrated male Yorkshire pigs of approximately 20 kg) which were associated with separate experimental studies that have been described in detail elsewhere [,]. The animals were infected through either direct intra-oropharyngeal (IOP) inoculation (FMDV A24 Cruzeiro, Case 1[]), or through direct contact exposure to previously infected pigs (FMDV O1 Campos, Case 2 []).
The two pigs developed severe clinical FMD at four (case 1) and two (case 2) days after virus exposure (dpe), with fever (>40°C), lameness and vesiculation at lesion predilection sites (including snout, oral cavity, heel bulbs and coronary bands). Both pigs died with clinical signs of congestive heart failure (respiratory distress and tachycardia) on the morning of the second day of clinical FMD infection (5 dpe for case 1; 3 dpe for case 2). Postmortem examinations of both pigs revealed multifocal regions of pallor on the epicardial surfaces, which extended into the myocardium when sectioned. No other abnormalities were observed.
Sample collection and analysis
Antibodies used for multichannel immunofluorescence microscopy
FMDV serotype A capsid []
FMDV serotype O capsid []
Wasington State University/PG2031
Monocytes, macrophages, granulocytes
Wasington State University/PBOV2037
CTLs and NK-cells
Large Mononuclear cells
Total Inflammatory cells
77.13 # /18.74
96.63 ¥ /19.16
8.38 # /1.61
28.88 ¥ /2.39
Two tailed P -value
Serum samples obtained prior to death (3 dpe) from case 2 (infected with FMDV O1 Campos) contained 5.50 log10 viral RNA copies/μl, and the highest viral load found in myocardial tissue samples was 6.46 log10 RNA copies/mg. Virus isolation on LFBK αvβ6 cells was positive for both serum and tissue samples. Myocardium had regions of myocyte degeneration and interstitial edema, comparable to that observed in case 1 (Figure 1C). Also similar to case 1, there was a mononuclear inflammatory reaction associated with regions of tissue damage, however, there were significantly fewer large mononuclear leukocytes (P = 0.0026) and total numbers of inflammatory cells (P = 0.0035) compared to case 1 (Table 2; Figure 1B-C). Inflammatory cells consisted mostly of spindle-shaped cells with elongated nuclei, in combination with small mononuclear cells (Figure 1C). The phenotypes of inflammatory cells were similar to those defined for case 1, despite a reduced abundance of cellular infiltrate (Figure 2D-F). For this pig, CD172a was utilized as a 2nd monocyte-lineage marker in combination with CD163. These cells were found in close proximity to infected cardiomyocytes, although, in contrast to observations in tissue sections from case 1, myophagocytic activity could not be clearly demonstrated. Similar to case 1, there was a distinct population of CD8+ cells (Figure 3C-D) without concurrent detection of CD3 or CD21 in serial sections.
The current report presents a descriptive investigation of the myocarditic properties of FMDV, based on analysis of myocardial tissue samples from two pigs that died during acute infection with FMDV A24 Cruzeiro or FMDV O1 Campos. There was a difference in the time elapsed from inoculation to death between the two cases (3 and 5 days respectively). However, both pigs died during the second day of clinical FMD. There are several potential contributors to the differences between the cases including variability of the viral strains used, host immune responses, and manner of exposure to FMDV (IOP inoculation vs. contact exposure). High levels of FMDV were detected by qRT-PCR and virus isolation in the myocardium of both pigs. For both pigs, myocardial levels of FMDV RNA exceeded those measured in serum although there was no serum available for analysis from the day of death for case 1. Histopathological evaluation of tissue sections from both cases indicated comparable extents of myocardial tissue damage, although with a substantially greater quantity of inflammatory cells in samples from the pig infected by FMDV A24 Cruzeiro. Since the cardiomyocyte degeneration is present with and without inflammation, this finding suggests that a substantial portion of the tissue damage may be a direct consequence of virus replication rather than a result of the host-response to infection. There was also a distinction in the morphology of inflammatory cells, with a greater abundance of large macrophage-like cells in case 1, in comparison to a relatively larger proportion of elongated cells (suspect dendritic cells) in case 2 (Figure 1B-C ). This indicates a continuum of potential cellular responses in FMDV-induced myocarditis; however, the causality of the variation in these cases was not determined in the current study.
The phenoptypic composition of the inflammatory infiltrates, as determined by the detection of specific leukocyte cell surface markers, was comparable across both cases. A large proportion of inflammatory cells present in myocardial sections from both cases were CD163+, which is consistent with both macrophages and tissue DCs []. However, the morphology of these presumptive monocyte-lineage cells varied across the two cases, with predominantly large mononuclear cells in case 1 and small elongated cells in case 2. The combinations of cell surface markers used for characterization of inflammatory cells do not enable clear distinction between DCs and cells of macrophage lineage. The overall findings suggest that the leukocyte populations present in both cases, were of similar histogenesis, but of different stages of maturation and/or activation. It was not possible to use the exact same combinations of cell markers for detection of cells of monocyte lineage across the two FMDV serotypes because of isotype differences in antibodies utilized for FMDV detection, (Table 1). Preliminary studies indicated that a large proportion of inflammatory cells in the myocardium of case 1 were also CD172a+, and that the distribution of these cells was similar to that of CD44+ (not shown). Phenotypic characterization of the inflammatory infiltrates in these two cases was comparable to reports of FMDV associated myocarditis in lambs [], although, in contrast to findings in this current study, the previous publication also reported detection of lymphocytes and plasma cells in myocardial sections.
There was a consistent lack of detection of CD3 and CD21 expression in inflammatory infiltrates, suggesting an absence of B- and T- lymphocytes, which may be related to the very early stages of infection at which these pigs died (3–5 days after infection). The CD8+/CD3-/CD21- phenotype, small mononuclear cells detected in myocardial infiltrates of both cases described herein is consistent with NK-cells []. The large quantity of these cells, particularly in case 1 suggests that NK-cells are involved in the pathogenesis of acute FMDV myocarditis in pigs. CD8+ cells were found in close proximity to FMDV-infected myocytes, but without intracellular localization of FMDV protein. This contrasts the colocalization of FMDV and actin within intracellular compartments of CD44+ cells (myophagocytosis, Figure 4). These observations are consistent with the cytotoxic functions of NK-cells versus the phagocytic role of macrophages.
Variation in the intensity of myocardial inflammation between different virus strains has previously been reported for Cocksackie B virus, a related virus within Picornaviridae []. Previous descriptions of the characteristics of myocardial injury in pigs caused by encephalomyocarditis virus (EMCV), another Picornavirus, are strikingly similar to the current findings [], suggesting mechanistic similarities between the two viruses and/or the associated host responses. A previous study investigating the pathogenesis of EMCV in pigs described a lympho-histocytic inflammatory response in infected myocardium, which increased progressively from 6 to 30 hours post infection []. This suggests that the difference in inflammatory intensity of the two pigs of the current study, may indicate that these two cases represents different phases of infection. However, the differences observed between the two cases may be a result of different characteristics of the two FMDV strains with which the pigs were infected.
Overall, the two cases of FMDV-associated myocarditis described herein had exceedingly similar clinical and pathological characteristics. There was substantial histomorphologic variation between the cases, but the mechanistic differences underlying this disparity could not be determined in this purely descriptive analysis. There is a need for more comprehensive studies to further investigate the pathogenesis of FMDV-associated myocarditis. Analysis of specific host-virus interactions involved in the onset and progression of myocardial injury, including comparative studies of related picornaviruses with more consistent cardiotropism, could help to further elucidate the mechanisms of this atypical, yet important variation of FMDV pathogenesis.
CS is a recipient of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. This research was funded in part by ARS-CRIS Project 1940- 32000-057-00D and an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security (award number HSHQDC-11-X-00189). George R Smoliga and Ethan J Hartwig processed samples. The authors acknowledge Erin B Howey for contributing to design of the immunofluorescence assays.
- Kandeil A, El-Shesheny R, Kayali G, Moatasim Y, Bagato O, Darwish M, Gaffar A, Younes A, Farag T, Kutkat MA: Characterization of the recent outbreak of foot-and-mouth disease virus serotype SAT2 in Egypt. Arch Virol. 2013, 158: 619-627. 10.1007/s00705-012-1529-y.View ArticlePubMedGoogle Scholar
- Gulbahar MY, Davis WC, Guvenc T, Yarim M, Parlak U, Kabak YB: Myocarditis associated with foot-and-mouth disease virus type O in lambs. Vet Pathol. 2007, 44: 589-599. 10.1354/vp.44-5-589.View ArticlePubMedGoogle Scholar
- Arzt J, Baxt B, Grubman MJ, Jackson T, Juleff N, Rhyan J, Rieder E, Waters R, Rodriguez LL: The pathogenesis of foot-and-mouth disease II: viral pathways in swine, small ruminants, and wildlife; myotropism, chronic syndromes, and molecular virus-host interactions. Transbound Emerg Dis. 2011, 58: 305-326. 10.1111/j.1865-1682.2011.01236.x.View ArticlePubMedGoogle Scholar
- Karapinar T, Dabak DO, Kuloglu T, Bulut H: High cardiac troponin I plasma concentration in a calf with myocarditis. Can Vet J. 2010, 51: 397-399.PubMed CentralPubMedGoogle Scholar
- Ryan E, Horsington J, Durand S, Brooks H, Alexandersen S, Brownlie J, Zhang Z: Foot-and-mouth disease virus infection in young lambs: Pathogenesis and tissue tropism. Vet Microbiol. 2007, 127: 258-274. 10.1016/j.vetmic.2007.08.029.View ArticlePubMedGoogle Scholar
- Donaldson AI, Ferris NP, Wells GA: Experimental foot-and-mouth disease in fattening pigs, sows and piglets in relation to outbreaks in the field. Vet Rec. 1984, 115: 509-512. 10.1136/vr.115.20.509.View ArticlePubMedGoogle Scholar
- Pacheco JM, Gladue DP, Holinka LG, Arzt J, Bishop E, Smoliga GR, Pauszek SJ, Bracht A, O'Donnell V, Fernandez Sainz I, Fletcher P, Piccone ME, Rodrigues LL, Borca MV: A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle. Virology. 2013, 446: 260-267. 10.1016/j.virol.2013.08.003.View ArticlePubMedGoogle Scholar
- Stenfeldt C, Pacheco JM, Rodriguez L, Arzt J: Infection dynamics of foot-and-mouth disease virus in pigs using two novel simulated-natural inoculation methods. Res Vet Sci. 2014, 96: 396-405. 10.1016/j.rvsc.2014.01.009.View ArticlePubMedGoogle Scholar
- Arzt J, Pacheco JM, Rodriguez LL: The early pathogenesis of foot-and-mouth disease in cattle after aerosol inoculation: identification of the nasopharynx as the primary site of infection. Vet Pathol. 2010, 47: 1048-1063. 10.1177/0300985810372509.View ArticlePubMedGoogle Scholar
- Arzt J, Gregg DA, Clavijo A, Rodriguez LL: Optimization of immunohistochemical and fluorescent antibody techniques for localization of Foot-and-mouth disease virus in animal tissues. J Vet Diagn Invest. 2009, 21: 779-792. 10.1177/104063870902100604.View ArticlePubMedGoogle Scholar
- LaRocco M, Krug PW, Kramer E, Ahmed Z, Pacheco JM, Duque H, Baxt B, Rodriguez LL: A continuous bovine kidney cell line constitutively expressing bovine alphavbeta6 integrin has increased susceptibility to foot-and-mouth disease virus. J Clin Microbiol. 2013, 51: 1714-1720. 10.1128/JCM.03370-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Piriou-Guzylack L, Salmon H: Membrane markers of the immune cells in swine: an update. Vet Res. 2008, 39: 54-10.1051/vetres:2008030.View ArticlePubMedGoogle Scholar
- Talker SC, Kaser T, Reutner K, Sedlak C, Mair KH, Koinig H, Graage R, Viehmann M, Klingler E, Ladinig A: Phenotypic maturation of porcine NK- and T-cell subsets. Dev Comp Immunol. 2013, 40: 51-68. 10.1016/j.dci.2013.01.003.View ArticlePubMedGoogle Scholar
- Cao Y, Schnurr DP, Schmidt NJ: Differing cardiotropic and myocarditic properties of group B type 4 coxsackievirus strains. Arch Virol. 1984, 80: 119-130. 10.1007/BF01310653.View ArticlePubMedGoogle Scholar
- Gelmetti D, Meroni A, Brocchi E, Koenen F, Cammarata G: Pathogenesis of encephalomyocarditis experimental infection in young piglets: a potential animal model to study viral myocarditis. Vet Res. 2006, 37: 15-23. 10.1051/vetres:2005041.View ArticlePubMedGoogle Scholar
- Baxt B, Vakharia V, Moore DM, Franke AJ, Morgan DO: Analysis of neutralizing antigenic sites on the surface of type A12 foot-and-mouth disease virus. J Virol. 1989, 63: 2143-2151.PubMed CentralPubMedGoogle Scholar
- Stave JW, Card JL, Morgan DO: Analysis of foot-and-mouth disease virus type O1 Brugge neutralization epitopes using monoclonal antibodies. J Gen Virol. 1986, 67: 2083-2092. 10.1099/0022-1317-67-10-2083.View ArticlePubMedGoogle Scholar
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